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Why does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?
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- Why does the oxidase test have to be read quickly?How is the oxidase test performed? Using Kovac’s reagent swabWhat is the unknownn organism based on this biochemical test results? Gram Negative Oxidase Positive Catalase Positive MAC: Lactose Fermenter MSA: Mannitol Fermenter Gelatin Hydrolysis: Negative Indole Production: Negative Motility Indole Ornithine Medium: Negative MR/VP Test: Negative Nitrate Test: Negative Simmon Citrate Test: Positive Urease Test: Positive Possible Organisms: Vibrio fischeri Enterococcus faecalis Micrococcus luteus Streptococcus pneumoniae Pseudomonas aeruginosa Proteus mirabilis Acinetobacter baumannii Staphylococcus aureus Mycoplasma pneumoniae Escherichia coli Candida albicans Salmonella enterica Listeria monocytogenes
- Why do the results for the confirmed test represent faecal coliform in the analysis of water using the MPN procedure?If the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitativeWhen using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of coli?
- Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a hospitalized patient with a urinary tract infection. The organism produced colorless colonies on MacConkey agar and gives the following biochemical reactions: PAD – positive H2S – positive positive Citrate – positive Ornithine positive Lysine – negative Indole - negative Urease –In the amino acid decarboxylase test (lysine decarboxylase), we added mineral oil to the tube before incubation. What is the purpose of the mineral oil? What is the indicator in the lysine decarboxylase media?How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible unknown? Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis. Create flow chart (dichotomues) using at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test.
- What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacteria can metabolize various carbohydrates? Explain how conducting the MR/VP test too soon in a culture’s growth cycle can lead to false negatives? Generally, the color change we see in these tests is a result of what change in the media? Explain. What is the MR-VP results for coli and E. aerogenes?Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is the reagent used in this test? What media is used? Describe the biochemical abilities of these organisms .