Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
Q: Briefly describe a method for the preparation of multilamellar vesicles.
A: Definition Multivesicular vesicles (MVVs) are vesicles-in-vesicles systems made from…
Q: Why does cyanide inhibit electron transport but not photosynthesis? O In animals cyanide binding to…
A: The correct option would be option 3) in animals cyanide binds to complex IV and prevents hydrogen…
Q: Draw the two possible Haworth structures (both alpha and beta anomers) for the following…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: II. Tests for Lipids A. Bromine Test Table 3. Results for Bromine test. SAMPLE Ranking of the number…
A: Hi. Thank you for the question. As per the honor code, we are allowed to answer three sub-parts at a…
Q: . FATS OR LIPIDS STARTS HYDROLYSIS IN THE STOMACH. THERE ARE TWO FAT SPLITTING ENZYMES ONE IN THE…
A: Macro molecules such as protein, Carbohydrates and Lipids are all taken in diet to provide energy to…
Q: How will I use my substrate R110 calibration curve in the trypsin enzyme lab? Can I use the…
A: Yes, you can use your R110 Calibration curve in Trypsin enzyme kinetic study to calculate the…
Q: fill in the blanks The two enzymes in glycolysis that use ATP to perform substrate phosphorylation…
A: Substrate-level phosphorylation produces ATP or GTP by directly transferring a phosphate group from…
Q: The number of ATP molecules that can be made from the oxidation of cis-11-pentatadecenoic acid…
A: The end products of complete oxidation of fatty acids is CO2 and water. It takes place via 2…
Q: Chemistry 1) Gluconeogenesis from pyruvate involves a simple reversal of all of the glycolysis…
A: "Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a…
A: The Bradford assay is based on the fact that the absorbance maximum for an acidic solution of…
Q: What factors in a population would mean that the Hardy-Weinberg principle does not apply?
A: Hardy-Weinberg principle states that, the allelic frequencies and genotypic frequencies in a…
Q: H druclose IS 2.5mM Gnd &0mM From letl→ to right monine@ 25°c what is the eneryy change? inK/mol
A: During a chemical reaction, in order for the reaction to take place, a few or all chemical…
Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml)…
A: The Bradford protein assay is a colorimetric assay using the Bradford reagent (acidified Coomassie…
Q: Tabulate the total number of ATP equivalents that would be produced by the metabolism of the…
A: The given phospholipid has glycerol, a sugar (glucose), a phosphate group, and two fatty acids…
Q: Chemistry A biomolecule has a K_d of 1.6mM. A mutated-version of the same biomoleucel was bound by…
A: [P] + [L] → [PL] Dissociation constant, Kd = [P] [L] / [PL] Dissociation constant (Kd) - is…
Q: DNA ladder 1 DNA ladder 2 bp ng/Sul 10000 8000 40 40 6000 3000 40 4000 40 700 600 3000 40 500 -2000…
A: Agarose gel electrophoresis is used to separate DNA molecules.
Q: 5. Indicate which one of the following statements is not correct: a. Drugs that are taken up into…
A: Drugs can be administered into the body via various sites; Orally, sublingual, intravenous, rectal,…
Q: Associate each of the following with either insulin or glucagon signaling. INSULIN GLUCAGON: No…
A: Glucagon and insulin are two major hormones produced by the exocrine cells of pancreas and…
Q: What test can be performed to identify this lipid structure?
A: A group of organic compounds includes lipids that are insoluble or poorly soluble in water.…
Q: Describe the movement of protons that is caused by the action of the mitochondrial electron…
A: Glycolysis, TCA cycle, and ETC all are interconnected processes. Respiration is an oxidative…
Q: 1. Relate the chemical structure of an enzyme to its specificity and catalytic activity. 2. Design a…
A: Biological catalysts are also known by the name enzymes. The enzyme is also present in our saliva.…
Q: Suppose an mRNA transcript with the following base sequence reaches a ribosome: 5'-…
A: A codon is a sequence of three consecutive nucleotides in a DNA or RNA that codes for a specific…
Q: Following on from the last question, what assays could you do to find out if your mitochondria are…
A: Succinate dehydrogenase is an essential enzyme that connects oxidative phosphorylation and the…
Q: Which description is true regarding beeswax
A: cera alba which is chemical name of beewax is a natural wax which is produced by honey bees. The…
Q: ill in the blank The carbohydrate polymer amylose has ( ) type of glucose linkages,…
A: Cellulose is the most abundant organic polymer on the earth. It is a polysaccharide composed of a…
Q: 11. ADULTS WHO CANNOT TOLERATE MILK OR MILK PRODUCTS LACK THE ENZYME? A. LACTASE B. ENTEROKINASE C.…
A: Since you have posted multiple questions we will solve the first question for you. If you want any…
Q: Place the stages of PCR in the correct order -Anneal -Denature -Analyse -Extend
A: Polymerase chain reaction (PCR) is a laboratory technique for rapidly amplifying DNA to produce…
Q: acid, protein, lipid or a carbohydrate. 1. CH,OH CH2OH CH,OH о н H H H H H H H H Он OH он он Он
A: Nucleic acid is the polymer of nucleotides, protein is the polymer of amino acids, carbohydrate…
Q: Ketogenesis Transamination Citric acid cycle Gluconeogenesis and deamination Glycogenesis Lactate…
A: All the events as mentioned in the table occur in a specific order. Initially the process of…
Q: There are 20 standard amino acids and only 10 major monosaccharides in eukaryotes, yet there are so…
A: The biological macromolecules can be classified as nucleic acids, proteins, carbohydrates and…
Q: Draw the Fischer projections representing the L forms of the following amino acids at pH higher than…
A: Amino acids contain amino group and carboxyl group along with R side chain. The R side chain defines…
Q: If one molecule of fructose-1,6-bisphosphate is converted to pyruvate how many molecules of ATP,…
A: The metabolic mechanism that transforms glucose to pyruvic acid is known as glycolysis. This free…
Q: The aldolase reaction cleaves fructose-1,6-bisphosphate to make G-3-P and DHAP. Draw the structures…
A: Aldolases are divided into two classes based on their reaction mechanism: class I and class II. The…
Q: The figure provided in in the introduction above shows adenine being deaminated to form the…
A: Wobble hypothesis - is about the base pairing between nitrogen bases in 1st and 2nd positions of the…
Q: Identify the 4 steps of gluconeogenesis that are different from glycolysis. Write the reactants,…
A: Gluconeogenesis (GNG) is a metabolic pathway that results in the generation of glucose from certain…
Q: Match the process with the cellular location in which it occurs. You may need to use the same…
A: The eukaryotic cell is compartmentalized with specific reaction occurring in specific compartments…
Q: What is an invasive species?
A: Invasive species can do all sorts of damage to an existing echosystem,like changing habitats and…
Q: 20-92 How many different primary structures are possible for a four-amino-acid segment of a protein…
A: The primary structure of a protein is composed of amino acids which are joined together through…
Q: Using skeletal structures, draw the structure of a simple triacylglyceride containing 1…
A: A triglyceride is an ester derived from glycerol and three fatty acids.
Q: The following is part of the non-template strand of DNA for a gene. 5'-TACTATCATGAGAGATAGGAG-3'…
A: Transcription is the process by which the information in a strand of DNA is copied into a messenger…
Q: 10. It was found that At coordinates to a metal in a 4:1 stoichiometry. Noting that A has a ba…
A: Biological important of coordination compounds like the heme group, which is Fe-Porphyrin complex…
Q: Describe how the structure of immunoglobulins is related to their function
A: Immunoglobulins- These are heterodimeric proteins composed of - 2 heavy (H) and 2 light (L) chains.
Q: For every 6 molecules of 3-phosphoglycerate produced by rubisco, _____ are converted to hexoses…
A: Calvin cycle is the second stage of photosynthesis, where the products of light reactions ATP and…
Q: what are fluid and electrolytes? how do they impact the body? what are the functions of fluid and…
A: The link between fluid intake and output is referred to as fluid balance. Fluid balance aims to keep…
Q: 1.Which of the following are cofactors/coenzymes required for fatty acid oxidation? Choose all…
A: Fatty acids are the source of large amount of energy. Fatty acids are degraded through the process…
Q: Why do people with dark skin at risk to have VitaminD deficiency?
A: Dark skin people have more melanin production in their skin.
Q: rank in order that releases most to least amount of energy adenosine triphosphate (ATP) acetyl…
A: Introduction: Organisms require energy for various activities like muscle contraction and cellular…
Q: Using the word anomeric carbon, “acceptor” nucleophilic substrate, and activated “donor”, describe…
A: In polysaccharides the monosaccharide units are linked by glycosidic linkages.
Q: 1. Considering the following nucleotide sequence in an mRNA molecule: 5’ AUG UUA CGU AAU GCU GUC…
A: The genetic code is a set of three-letter combinations of nucleotides that corresponds to a specific…
Q: Identify the name of the amino acid and its group (aliphatic, aromatic, acidic, basic, hydroxylic,…
A: 1. The 1st aminoacid is Proline. Proline; Neutral nonpolar; Non-essential Explanation: Proline…
Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
Step by step
Solved in 2 steps
- how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a hospitalized patient with a urinary tract infection. The organism produced colorless colonies on MacConkey agar and gives the following biochemical reactions: PAD – positive H2S – positive positive Citrate – positive Ornithine positive Lysine – negative Indole - negative Urease –What concentration of agar is used in a semisolid medium for motility determination? How does that compare to a typical solid medium ? Explain.
- WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE MEDIA COLOR CHANGE?Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is the reagent used in this test? What media is used? Describe the biochemical abilities of these organisms .
- name the genus and species of 2 enteric bacteria that do not ferment lactose discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayWhat is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacteria can metabolize various carbohydrates? Explain how conducting the MR/VP test too soon in a culture’s growth cycle can lead to false negatives? Generally, the color change we see in these tests is a result of what change in the media? Explain. What is the MR-VP results for coli and E. aerogenes?
- Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/LAgar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.