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5
What are the cofactors are involved in
A) FAD
B) CoA
C)NAD+
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- Give a complete and well descriptive definition of the following:1.1 Fractional saturation1.2Allostery1.3 Metal activated enzymes 1.4 Metalloenzymes1.5 Acid-base catalysisWhere do each of these 5 main themes occur in the chymotrypsin mechanism? 1) substrate specificity 2) induced fit 3) covalent catalysis 4) acid/base catalysis 5) transition state stabilizationGiven the active site diagram below, indicate the mechanism(s) of catalysis. 5 HO OH HN OH ΝΗ *HN ΝΗ Zn²+ Acid-base, Metal ion 3 Metal ion, By Approximation Metal ion Acid-Base, By Approximation Acid-base
- 8. At room temperature (20 °C), milk turns sour in about 64 hours. In a refrigerator at 3 °C, milk can be stored three times as long before it sours. a) Estimate the activation energy of the reaction that causes the souring of milk. b) How long should it take milk to sour at 40 °C?21. You want to maintain pH = 7.0 for an enzyme-catalyzed reaction that will produce hydrogen ions along with the desired product. At equal concentrations, which weak acid, if any, will serve as the better buffer for the reaction: acid A, with pk₁= 6.5, or acid B, with pk=7.5? A) acid A B) Water is as good as either of the acids available. C) acid B D) Both are equally effective.From the active site diagram below, please indicate the cofactor from the indicated components. HO 4 2 3 5 1 OH HN 5 NH *HN ΝΗ OH OOH Zn²+ 2 3
- 1. In a catabolic pathway, metabolite X gets converted into metabolite Y, metabolite X 0 J mol-¹ and AG' is <0 J mol-¹ for this reaction. a) At standard states, is this reaction favorable? b) Is the reaction favorable or unfavorable at cellular condition? c) Is this an endergonic or exergonic reaction in the cell? d) Would you describe this as a thermodynamically downhill or uphill reaction in the cell?4. The enthalpy change of a chemical process depends only on the initial and final conditions, it is independent of the number of intermediate steps in the process. This sums up the ... a) Hess law b) heat additivity law c) law of the enthalpy of formation d) a and b e) a and c f) b and c 5. The reverse activation energy of a certain exothermic reaction is 50 kJ / mol. The direct activation energy of this reaction is: a) greater than 50 kJ / mol b) less than 50 kJ / mol c) 50 kJ / mol d) 0 kJ / mol 6. What is the overall reaction order for the next reaction. 2A + B – 2C + D v = k [A]• [B]* b) 4 c) 3 d) 2 e) 1 5432N119. Define the terms “cofactor” and “coenzyme.” 20. What is the difference between general acid-base catalysis and specific acid-base catalysis? (Assume that the solvent is water.)
- 1. Answer the following questions, based on this reaction: S +E ES EP E +P A)What is the slow step of this reaction, and why is that step slow?Show reaction mechanisms involving a coenzyme by (a) transfer reaction (b) redox reaction14) Which of the following statements is true under the conditions provided: the enzyme concentration is 0.5 nM, substrate concentration is 10 µM, and the KM = 20 µM? a) The enzymatic reaction occurs at maximal velocity. b) The enzymatic reaction occurs between half-maximal and maximal velocity. c) The enzymatic reaction occurs at around half-maximal velocity. d) The enzymatic reaction occurs at between zero and half-maximal velocity. e) Not enough information given to know about the enzymatic reaction rate.