Used a compound microscope measured the actual length of trichnoympha. 400x magnification. “What is the value of each space under this magnification?”
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- Describe the microscope in 2-3 sentencesAssume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.Why is plate count more sensitive than a microscopic count?
- A lab revolving around compound microscopes was conducted. Please answer the following questions (questions 1, 2, and 3). If a specimen (placed on a slide) has little or no color, what level of light intensity should you use; should the intenisty of the light be high or low (choose)? Which objective should be in position before you start to focus the microscope? Which focus knob should you use first with this objective?Write the term that the phrase describes. 1. Large knob that moves the stage or objective lens a great distance. Used with scanning or low-power objective lenses only. 2. Flat platform beneath the objective lens on which the microscope slide is placed. 3. Removable lenses that you look through to observe the microscope slide. 4. Small knob that moves the stage or objective lens a very small distance and is used for precision focusing. 5. Extends from the arm and contains the ocular lenses and rotating nosepiece. 6. Lens that condenses light through the specimen and is located below the stage. 7. Light from specimen passes through these lenses first. These lenses are located in the rotating nosepiece. 8. Wide bottom part that supports the microscope. 9. Regulates the amount of light passing through the condenser. 10. Vertical portion that connects the base to the head.The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1). Identify B (name of the structures arrows point to; blank 2) - Identify C (name of the structures arrows point to; blank 3) High mag low mag high mag Blank # 1 Blank # 2 Blank # 3
- a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.A question from histology. a) Explain the term HE staining b) how many preparations are there for LM? Please answer all parts if possible.D) When performing fluorescence microscopy what are the stokes shift and why is it better to have fluorochromes with a large stokes shift? E) What is photobleaching and what is done when imaging histological samples to overcome it when performing fluorescence microscopy?
- Using a diagrammatical presentation show how serial dilution was performed if the final volume in each tube was 5ml.a. Why must you slowly turn the fine adjustment knob? b. Is natural light or is artificial light used when using the microscope?If both plates were accidently incubated at 37°C, how might interpretation of the results change? Explain.