The lac operon contains three genes: lacZ, lacY, and lacA for ß-galactosidase, galactoside permease, and thiogalactosidetransacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the Igene for the Lac repressor protein and a promoter for that gene.regulatory geneDNAP₁ lacl geneThe lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, anisomer of lactose, is the signal molecule that binds to the Lac repressor.P OIncrease in gene productsThe I gene is disabled.Lactose is present in the cell.The Lac repressor dissociates from DNA.lac operonlacZ geneDetermine which events will increase the concentration of lac gene products and which will decrease the concentration of lacgene products.Allolactose is not present in the cell.lacy genelacA geneDecrease in gene productsAllolactose binds to the Lac repressor protein.The Lac repressor remains bound to the operator.

Lac operon is a model of gene regulation system in prokaryotes. it consists of three genes , lac Z,…

Answered: The lac operon contains three genes:…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Mutations in the genes of the lac operon might affect the regulation of β-galactosidase synthesis. For each mutation listed below, indicating whether β-galactosidase would be regulated normally, always ON or always OFF. Mutation in operator site prevents repressor binding Mutation in lacIgene prevents repressor from binding operator Mutation in lacIgene prevents repressor from binding allolactose Nonsense mutation in lacZgenere
The molecules indicated below can be used experimentally due to their roles in relation to the expression of the lac operon. Drag the listed molecules to their corresponding bin. Reset Help ONPG IPTG X-Gal allolactose Dragable beta-galactosidase cleaves this molecule to form a visible yellow product. beta-galactosidase cleaves this molecule which then dimerizes to form a visible blue product. This molecule induces the transcription of the lac operon by inactivating the lac repressor.
Indicate whether each of the following statements about the lac operon are true or false: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the genes A constitutive Lac+ phenotype could mean either that the production of repressor is defective or that the operator sequence is defective The lacl gene is next to, but is not part of, the lac 1. TRUE operon 2. FALSE Bacteria with a lac* phenotype cannot grow on media containing lactose as the only sugar The structural genes of the lac operon are each transcribed as a separate MRNA Expression of the Lac Repressor from the lacl promoter is regulated by lactose
Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence of glucose when other metabolic operons continue to be sensitive to the absence of glucose.
Which of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose? Group of answer choices I+ O+ Z+ Y+ A+ I- O+ Z- Y- A- I+ OC Z+ Y+ A+ IS O+ Z+ Y+ A+ I+ O+ Z- Y+ A+
IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Based on your knowledge of the regulation of the lac operon, predict with explanation whether these gene products (B-galactosidase, lactose Permease) will be produced. a IPOZY IPOZY b. IP'O'Z'Y/IPOZY" +IPTG B-galactosidase -IPTG Permease B-galactosidase Permease Only typed answer. Will upvote you immediately. Please help
Describe the binding of RNA polymerase, repressors, andactivators to the lac operon when both lactose and glucose are scarce. What is the effect of these scarcities ontranscription of the lac operon?
A theoretical operon (theo) in E. coli contains several structural genes encoding enzymes that are involved sequentially in the biosynthesis of an amino acid. Unlike the lac operon, in which the repressor gene is separate from the operon, the gene encoding the regulator molecule is contained within the theo operon. When the end product (the amino acid) is present, it combines with the regulator molecule, and this complex binds to the operator, repressing the operon. In the absence of the amino acid, the regulatory molecule fails to bind to the operator, and transcription proceeds. Characterize this operon, then consider the following mutations, as well as the situation in which the wild-type gene is present along with the mutantgene in partially diploid cells (F¿):(a) Mutation in the operator region.(b) Mutation in the promoter region.(c) Mutation in the regulator gene.In each case, will the operon be active or inactive in transcription, assuming that the mutation affects the…
Describe the main functional differences between the TRP and the Lac operons. Main functional differences DO NOT refer to the fact that one operon involves TRP and the other involves Lac. They also don’t include that there are different genes found in the TRP operon compared to the Lac operon or that the repressor in the Lac operon is called Lac l. Also DO NOT simply list how both operons work to answer this question.
When referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, transcription termination at the attenuator region is likely the ribosomes stall during translation of the attenuator region translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels tryptophan inactivates the repressor protein
Given the following mutant and conditions, predict the expression of the lacZ gene (no expression, basal level, activate level of expression). A mutant of E. coli has a mutation in the promoter of the lac operon that prevents RNA polymerase from binding. + glucose, - lactose + glucose, + lactose – glucose, + lactose
Strain ROFL4 has a premature stop mutation in the lacZ gene, resulting in a nonfunctional b-galactosidase. Otherwise all other parts of the operon are functional. 1. Can strain ROFL4 metabolize lactose if it is present? 2. If lactose is absent, will strain ROFL4 transcribe its lac operon? 3. If lactose is present, will strain ROFL4 transcribe its lac operon?

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