The isoelectric point of a polypeptide or protein is equal to the pH at which the overall charge is equal to zero (0). 1 i Find the pH range to within 1 pH unit over which the polypeptide below will have an overall charge of 0. (In class on Friday, I worked out the changes in charge that the N-terminal -NH3+ and the C-terminal -COOH groups undergo over a limited pH range. Follow through to apply the same approach to all of the ionizing or dissociating groups, including the N-terminal and C-terminal groups, to estimate their separate, individual contributions to the overall charge on the polypeptide as a function of pH. 6, identify the two residues that control the transition for the polypeptide to acquire an overall charge of 0.) Alanyl-cysteinyl-lysyl-glutamyl-asparaginyl-histidinyl-tyrosine Ala - Cys A C *H3N. CH3 H SH - Lys - Glu - Asn His - Tyr K E N H Y HO. H H ■NH3* | NH₂ HNT NH H Гон JOH Ka values 7.0 8.4 10.5 4.1 6.0 4.0 10.5 ΣZavg
The isoelectric point of a polypeptide or protein is equal to the pH at which the overall charge is equal to zero (0). 1 i Find the pH range to within 1 pH unit over which the polypeptide below will have an overall charge of 0. (In class on Friday, I worked out the changes in charge that the N-terminal -NH3+ and the C-terminal -COOH groups undergo over a limited pH range. Follow through to apply the same approach to all of the ionizing or dissociating groups, including the N-terminal and C-terminal groups, to estimate their separate, individual contributions to the overall charge on the polypeptide as a function of pH. 6, identify the two residues that control the transition for the polypeptide to acquire an overall charge of 0.) Alanyl-cysteinyl-lysyl-glutamyl-asparaginyl-histidinyl-tyrosine Ala - Cys A C *H3N. CH3 H SH - Lys - Glu - Asn His - Tyr K E N H Y HO. H H ■NH3* | NH₂ HNT NH H Гон JOH Ka values 7.0 8.4 10.5 4.1 6.0 4.0 10.5 ΣZavg
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.49P: 22-49 Based on your knowledge of the chemical properties of amino acid side chains, suggest a...
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