Question 2. An enzyme was found to convert Acetyl-coA to an acetyl-cysteine intermediate of the enzyme. The structure of COA is: H H НО СН, HS — СH2— СH, — N—С— СН, — СН;—N—С Ċ–Ċ–CH2-0 0-P-0-CH2 Adenine H CH5 H H H H Phosphopantetheine group of coenzyme A 2-O,PO OH Draw a plausible chemical mechanism. Where is the acetyl-group in acetyl-coA? What is the reactive group of the protein? The reaction is enhanced at higher pH. How is this consistent with your mechanism? Is covalent catalysis involved? www

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter18: Glycolysis
Section: Chapter Questions
Problem 24P: Based on your knowledge of the structure of NAD+ and an assumption that coenzyme dissociation is the...
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Question 2. An enzyme was found to convert Acetyl-coA to an acetyl-cysteine intermediate of the
enzyme. The structure of cOA is:
H
H
НО СН;
HS — СH2— СH2 — N—C—CH,— CH2—N- С—С—С—CH;—0—Р-
0–CH2
Adenine
н CH
H
H
H
Phosphopantetheine group of coenzyme A
2-O3PO
ОН
Draw a plausible chemical mechanism. Where is the acetyl-group in acetyl-coA? What is the reactive
group of the protein? The reaction is enhanced at higher pH. How is this consistent with your
mechanism? Is covalent catalysis involved?
Transcribed Image Text:Question 2. An enzyme was found to convert Acetyl-coA to an acetyl-cysteine intermediate of the enzyme. The structure of cOA is: H H НО СН; HS — СH2— СH2 — N—C—CH,— CH2—N- С—С—С—CH;—0—Р- 0–CH2 Adenine н CH H H H Phosphopantetheine group of coenzyme A 2-O3PO ОН Draw a plausible chemical mechanism. Where is the acetyl-group in acetyl-coA? What is the reactive group of the protein? The reaction is enhanced at higher pH. How is this consistent with your mechanism? Is covalent catalysis involved?
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