Question 1 Which of the following statements is true regarding compounds of the TCA cycle resulting from introduction into the TCA cycle of Glutamate labeled in the R group carboxyl carbon transaminated to a-Ketoglutarate and taken through one complete round back to Succinyl CoA? Multiple answers: Multiple answers are accepted for this question Select one or more answers and submit. For keyboard navigation. SHOW MORE V After one complete round Succinyl CoA will be radioactive. The labeled R group carboxyl of Glutamate will be released as radioactive carbon dioxide. The transfer of Acetyl-CoA to Oxaloacetate will be result in radioactive Citric Acid. d. A possible source of radioactive carbon dioxide is the Isocitrate Dehydrogenase reaction. A possible source of radioactive carbon dioxide is the a-Ketoglutarate Dehydrogenase reaction. No released carbon dioxide is radioactive. If taken through two complete rounds Succinyl CoA produced will be radioactive. The carbon of the resulting citric acid bearing the hydroxyl group is oxidized by a-Ketoglutarate Dehydrogenase. i Both of the - CHz- carbons of the formed Succinate are oxidized by Succinate Dehydrogenase

Question 1 Which of the following statements is true regarding compounds of the TCA cycle resulting from introduction into the TCA cycle of Glutamate labeled in the R group carboxyl carbon transaminated to a-Ketoglutarate and taken through one complete round back to Succinyl CoA? Multiple answers: Multiple answers are accepted for this question Select one or more answers and submit. For keyboard navigation. SHOW MORE V After one complete round Succinyl CoA will be radioactive. The labeled R group carboxyl of Glutamate will be released as radioactive carbon dioxide. The transfer of Acetyl-CoA to Oxaloacetate will be result in radioactive Citric Acid. d. A possible source of radioactive carbon dioxide is the Isocitrate Dehydrogenase reaction. A possible source of radioactive carbon dioxide is the a-Ketoglutarate Dehydrogenase reaction. No released carbon dioxide is radioactive. If taken through two complete rounds Succinyl CoA produced will be radioactive. The carbon of the resulting citric acid bearing the hydroxyl group is oxidized by a-Ketoglutarate Dehydrogenase. i Both of the - CHz- carbons of the formed Succinate are oxidized by Succinate Dehydrogenase

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 20P: Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have...

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Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have serious consequences for humans For example, defects in the gene for pyruvate kinase can result in a condition known as hemolytic anemia. Consult a reference to learn about hemolytic anemia, and discuss why such genetic defects lead to this condition.
A molecule -X inhibits oxidation of stearoyl-CoA by isolated mitochondria but has no effect on palmitoyl-carnitine oxidation, explain.
19) Consider the table of allosteric effectors and their effect on the following metabolic processes. Glycolysis Gluconeogenesis PDH complex Krebs cycle acetyl-CoA Not applicable AMP Not applicable + glucagon Not applicable Not applicable - Each row contains one error. Correct the error in the table below Table 2 Correction (indicate process and effect (+ or -) acetyl-CoA AMP glucagon - Briefly explain the effect of each allosteric effector on the corrected pathway (based on Table 2 above)
QUESTION 10 Select a property that does not belong to allosteric enzymes. They conform to hyperbolic Michaelis-Menten kinetics They may have binding sites for regulatory molecules that are separate from active sites They tend to have a signmoidal curve of rate versus [S] They undergo conformation changes as a result of modulator binding.
Question 2. Answer the following questions: A. The following experimental data was collected during a study of the catalytic activity of anintestinal peptidase with the substrate glycylglycine. Plot the data as a graph, and use it toestimate the Km and the Vmax for this enzyme. B. Now transform this data to plot it as a straight line (Lineweaver-Burk plot). Determine Km andthe Vmax for this enzyme using this new plot. Do your results agree with the estimates made fromthe first graph of the raw data (from 2A)? C. Now assume that the activity of this intestinal peptidase is regulated by covalent modificationof its catalytically active amino acid. Upon phosphorylation, the Km of the catalyzed reaction has been observed to increase by a factor of 3 without any effect on its Vmax. Is the enzyme getting activated or inhibited upon phosphorylation? Justify your answer. D. How will the Lineweaver-Burk plot of the phosphorylated enzyme differ from the plot of the unmodified enzyme (from 2B)?…
In the clinical case scenario provided, match the defective enzyme in Column A with the consequence of this enzyme defect in Column B. IND OAD DH Autopsy results from individual X showed normal levels of glucose and pyruvate, very low levels of NAD+ and very high levels of NADH. Their blood oxygen levels were normal, although the cause of death was given to be a lack of oxygen. Option A B C D Defective enzyme Phosphofructokinase Pyruvate dehydrogenase Cytochrome oxidase Lactate dehydrogenase write your answer here Consequence of this enzyme defect Impaired ATP production Impaired NAD production Impaired respiration Impaired oxygen I L OORD ORD OND DORO G
The citric acid cycle itself, which is composed of enzymatically catalyzed steps, can be thought of essentially as a supramolecular enzyme. Explain.
Please answer the following questions 1. Give the substrate for each of the following enzymes: a. dihydrofolate reductase b. acetylcholinesterase c. lactate dehydrogenase d. DNA polymerase e. aspartate transaminase
One of the regulators of the TCA cycle is succinyl CoA. Discuss the rationale for this molecule to be used to regulate the TCA cycle [include chemical structures and chemical equations where appropriate]. What is an allosteric inhibitor? How does it operate? For what TCA enzymes does succinyl CoA act as an inhibitor? What is the metabolic role of succinyl CoA? So then why is this molecule a reasonable choice as an inhibitor of the TCA?
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Lactate dehydrogenase is a tetramer of MW 134000 g/mole composed of subunits which are equal in size. It is found in the cytoplasm of eukaryotic and prokaryotic cells. Describe the secondary, tertiary and quaternary structures of lactate dehydrogenase.
Aminolevulinate (ALA) synthase is a pyridoxal phosphate containing enzyme displaying a non-sequential (ping-pong) enzyme mechanism. It catalyses the reaction: glycine + succinyl-CoA ⇌ aminolevulinate + CO2 (A) Sketch the Lineweaver Burke plots for ALA synthase varying glycine at three (3) different fixed concentrations of succinyl-CoA. Label the axes and the lines, and explain in words what you expect to see for these three lines. (B) Draw a Cleland diagram (diagram illustrating when the substrates and products form an enzyme substrate complex) for this reaction if glycine is the first substrate to bind.