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- TRUE or FALSE only! 1. Pure cultures contain only a single species and no other organisms. 2. Pour plate method is done by pipetting liquified warm agar into a petri dish containing an inoculum. 3. Flaming the test tube’s mouth is necessary so that the moisture that accumulated will evaporate. 4. After Gram staining, the Gram positive bacteria will appear blue while the Gram negative bacteria will appear red.Idenitfy the statements whether they are TRUE or FALSE 1. You should use P-20 micropipettes to transfer volumes for spread plating. 2. You should use P-200 micropipettes to transfer volumes for pour plating.TRUE or FALSE only! 1. The most important step in doing a slide mount technique is fixing the sample with heat or chemicals. 2. Diluted samples from the serial dilution procedure are used for pour plating and spread plating methods which can be done simultaneously. 3. In doing aseptic techniques, it is practiced to touch the tip of the inoculating loop to test its coolness for transfer of cultures. 4. One of the important points to consider when calibrating an ocular micrometer is that its lines must properly superimpose with that of a stage microruler.
- TRUE OR FALSE 1. The antheridium of a moss could be seen as a whole mount at 100x magnification. 2. The fine adjustment knob sharpens the image in all objectives. 3. While disinfecting the isolation room with UV light, stay inside so that your hands and clothes will also be sterilized.ク94% P 9:12 docs.google.com a * ZAIN IQ Iı. component used as a solidifying. . -1 * agent for media إجابتك media are used when specimen . . -2 .cannot be cultured soon after collection إجابتك media which can be used for. . -3 * motility testing إجابتك staining used for Mycobacterium. .-5 tuberculiosis3. Fill in the values below (0.5) 1 ml Sample Tube dilution Final dilution 1 ml sample 1 ml 1 ml TEED 9 ml 9 ml diluent diluent Tube dilution Final dilution 50 μl Sample 4. Fill in the values below (0.5) 9 ml 50 μl sample diluent 100 μ. 50 μl 0000 100 μl 100 μl diluent diluent diluent 50 μl 1ml 50 με 9 ml diluent 100 μl diluent
- Given Test Reagent Positive or Negative Result Reason/Explanation Ribose Bial’s Orcinol Test Maltose Benedict’s Test Milk Molisch Test Cellulose Iodine Test1. Fill up the table below to summarize the confirmatory test for casein and whey. Test Sample Filtrate Residue / Precipitate Milk Component Reagents Observations Inferences 2. Test for Milk Fat Briefly describe how milk fat is being tested?Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.
- If the concentration of solutes in the fluid surrounding the cell is greater than the cell's cytoplasm then the surrounding fluid is to the cell cytoplasm. 00000 Endergonic Exergonic Hypertonic Isotonic HypotonicAnswer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.Citrate test What is the color of the medium before inoculation? Green or blue
