All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.4: The Gram Stain
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Answer TRUE OR FALSE only.

 

1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium.

2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria.

3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure.

4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms.

5. Label all reagents with its name, concentration and date of its preparation except for water.

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