One of the buffers that can be used for running an agarose gel is 1x TBE (tris borate EDTA) buffer. The recipe for 1x TBE is: • 0.13 M tris • 45 mM boric acid • 2.5 mM EDTA How will you make 1 L of this buffer from 1 M stock solutions of tris, boric acid, and EDTA? Put your answers in the blanks. To make 1 Lof this buffer, would need: Lof 1 M Tris ml of 1 M boric acid ml of 1 M EDTA Then, these will be topped up with distilled water in a volumetric flask up to the 1 L mark.

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Acid Base Titration

An acid-base titration is the method of quantitative analysis for determining the concentration of an acid and base by exactly neutralizing it with a standard solution of base or acid having known concentration.