Of the 220 HIV-infected outpatients of the dermatological and venereological dispensary examined in 12, a positive result was obtained. The study was performed by solid-phase ELISA on polystyrene tablets to detect antibodies to HIV. Stage II serological diagnosis was applied to those who tested positive for ELISA to definitively establish the fact of HIV infection. There were 8 such persons. What is the principle of the method used in the first stage of serological diagnosis? Antibodies to which components of HIV are determined in ELISA? What is the diagnostic value of ELISA? What components are needed for the implementation of solid-phase ELISA? Which method should be used in the second stage of HIV detection to avoid overdiagnosis? What is the principle of immunoblotting? Antibodies to which components of HIV are determined by immunoblotting? According to the results of which serological method is the final diagnosis of HIV infection made?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Of the 220 HIV-infected outpatients of the dermatological and venereological

dispensary examined in 12, a positive result was obtained. The study was performed

by solid-phase ELISA on polystyrene tablets to detect antibodies to HIV. Stage II

serological diagnosis was applied to those who tested positive for ELISA to

definitively establish the fact of HIV infection. There were 8 such persons.

What is the principle of the method used in the first stage of serological diagnosis?

Antibodies to which components of HIV are determined in ELISA? What is the

diagnostic value of ELISA?

What components are needed for the implementation of solid-phase ELISA?

Which method should be used in the second stage of HIV detection to avoid

overdiagnosis?

What is the principle of immunoblotting? Antibodies to which components of HIV

are determined by immunoblotting?

According to the results of which serological method is the final diagnosis of HIV

infection made?

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