In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence of salt and protein?
Q: Describe how the following properties affect the function of a protien: A.) R group orientation B.) ...
A: Amino acids are biomolecules that are comprised of two functional groups, these are an amino group (...
Q: What are the 4 levels of structural organization of proteins and what are the important interactions...
A: The four levels of structural organization of proteins are : Primary structure, Secondary structure,...
Q: An athlete uses ATP (another chemical) as fuel for a sprint when a glucose molecule is broken down. ...
A: ATP is fundamental source of energy in humans and during sprinting we gets this ATP from different s...
Q: You sequence a new tRNA and discover that the anticodon sequence is 5'-IGC-3. What amino acid does i...
A: I corresponds to an unusual bas hypoxanthine, which is found in tRNA
Q: Chemically, Olestra is made of a six-carbon backbone and six fatty acid attachments. No nutrients ar...
A: Olestra- Indigestible fat and composed of 6 fatty acid attachments.
Q: Complete the procedure for the synthesis of aspirin by providing the answer to the blanks. In a 125-...
A: In folk medicines and over history people have used many compounds extracted from nature to cure var...
Q: Given the following question on the image identify the following:1. Total number of acetyl coA molec...
A: Maltose is a carbohydrate and it gets oxidized through glycolysis in cytoplasm and through the TCA c...
Q: Q30. During passage through the G1/S transition, the phosphatase that removes the Tyr15 phosphate fr...
A: Cyclin dependent kinases (CDKs) are enzymes which belongs to the family of protein kinases and invol...
Q: In cells, certain hormones, such as epinephrine, have the ability to raise the concentration of cAMP...
A: The ability of a protein to bind to a DNA segment can be analyzed using the method of Electrophoreti...
Q: Connection between Electron Transport & Phosphorylation Task: 1. Define P/O ratio 2. Explain its...
A: Our body is always working, continuously doing various metabolic activities even when we are sleepin...
Q: How to use the sterile filter? Show the different sizes and specifications of sterile filters.
A: Sterilization is the method that is used to eliminate all forms of life and biological agents presen...
Q: Reset Double-helical DNA Sugar-phosphate backbone (RNA) Base uracil in Phosphate group Single-strand...
A: S.No. DNA RNA 1 Deoxyribonucleic Acid. Ribonucleic Acid. 2 Generally double stra...
Q: What is the importance of culture media in the diagnosis of plant disease?
A: Plant disease: A disease is defined as any type of injurious abnormality and it is the pathogen tha...
Q: Functional groups and linkages assignment Complete the reactions below. Circle and identify all func...
A: The hydrocarbon compound is N-butylpropanamide which is reacting with water.
Q: Define the term isomer and distinguish among the three principal isomer types.
A: Molecules are composed of atoms. Atoms are the smallest particles of an element that can exist. Atom...
Q: What is the term applied to the trinucleotide shown by the arrow? 5' AU Ру AGGCC G C G G G ACCACCUGe...
A: This a structure of tRNA, The tRNA molecule has a distinctive folded structure with three hairpin lo...
Q: 5' AU Py U A AGGCC G. UGGC G C G GGG What modified nucleoside base is indicated by the arrow? dihydr...
A: The answer of the above question is : Pseudouracil (Ψ). Pseudouridine (psi- Ψ)is an isomer of the nu...
Q: Explain why all mono- and disaccharides are soluble in water? What are some examples of artificial s...
A: All mono- and disaccharides are soluble in water. Monosaccharides are glucose, fructose, and galacto...
Q: What are the disadvantages of using genetic engineering to obtain resistant plants?
A: The new genetic material (DNA and genes) has been transfecting or reprogramming cultured ce...
Q: How do I convert from DPM to picomoles per milligram of protein (pmol/mg)? I have the specific activ...
A: DPM refer to Disintegration per minute for a Radioactive decay. When a compound is labeled with a ra...
Q: In First order, the rate of reaction would be . ., if the concertation of an enzyme is increased by ...
A: The reaction may be classified according to the order of reaction, which is the number of reacting s...
Q: 1. What are the numbers of carbon atoms derived from the first acctyl-CoA that binds to fatty acid s...
A: Fatty acid synthase complex bind to Acetyl-CoA and other substrate and combine the C-atom backbone i...
Q: A protein P has two identical binding sites for a molecule A. The shape of the protein is dependent ...
A: The Scatchard Plot for a protein-ligand association help to determine the affinity and number of the...
Q: Evaluate the special traits of microbes in bioremediation of oil spill and their mechanism in degrad...
A: Process of removal of hramful/toxic substance using microbes or plant is called Bioremediation. Such...
Q: What is the role of ATP in cell signaling in biochemistry?
A: ATP has key functions in cell signaling and the signal transduction process heavily relies on ATP. A...
Q: Kingdom Type of Cells Number of Cells How it Obtains Reproduction Food Asexual by binary fission, bu...
A: Eukaryotes are organisms whose cells contain a nucleus (along with other membrane-bound organelles. ...
Q: Does this protein absorb light at 280 nm? If yes, please write (as a number) how many residues contr...
A: Amino acids are organic compound with functional group namely carboxyl and amino. Proteins are forme...
Q: A) illustrate in molecular detail how hemoglobin's reduced oxygen affinity is caused by protonation ...
A: In oxygen-breathing animals, hemoglobin (Hb) has developed as an efficient transport protein that tr...
Q: According to the configuration of the isostatic, syndiotactic and atactic polymers. Define each of ...
A: TACTICITY :- The steric arrangement in polymers is called tacticity.
Q: Help with #4 thanks
A: Enzymes are proteins that aid in the speeding up of metabolic reactions. Certain chemicals can slow ...
Q: E. coli ribonuclease H1 is an enzyme that catalyzes the hydrolysis of phosphodiester bonds in RNA. I...
A: Carboxylate relay One of the carboxylates ( Asp70) activates water molecule to release the hydroxyl ...
Q: Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa. Thirt...
A: Given Values: Molecular weight obtained from gel-filtration experiment = 130 kDa Weight of the fluor...
Q: How many different components make up a nucleoside? 4 2 3 5
A: Nucleosides are glycosylamines that are phosphorylated to form nucleotides.
Q: Which of the following cells is solely dependent on glucose as energy source? Muscle cells Kidney ce...
A: Inside the body, varying energy sources get exploited by different cells of various organs and tissu...
Q: Consider the following free energy diagram for an uncatalyzed and enzyme-catalyzed reaction. Select ...
A: Those proteins or biological catalysts which help to speed up the chemical reaction are termed enzym...
Q: Why is biochemistry called the chemistry of life?
A: The question is all about the subject biochemistry that it is called as chemistry of life as our hum...
Q: Which of the following is true of DNA and RNA? both are found in the chromosomes neither DNA n...
A: DNA is the genetic material in an prokaryotic and eukaryotic cell.
Q: Below is the primary sequence of a viral protein. MSVVNTEIKFPTHLRSGDFAIIDGMVVEVTSVEYKPVEQAVYLKYRYHL...
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
Q: What is the concepts of the native conformation of proteins? Why and how do proteins refold and unfo...
A: Proteins are polypeptide structures consisting of one or more long-chain amino acid residues. They a...
Q: Which of the following is NOT a dissimilarity between RNA and DNA? a) Types of bases b) Double-str...
A: RNA , DNA : Nucleic acids. RNA : Ribonucleic acid DNA : Deoxyribonucleic acid Both are structurall...
Q: Low-resolution X-ray diffraction analysis of a protein composed of long stretches of the sequence (-...
A: Fibroins are insoluble proteins that are present in silk produced by insects, such as the larvae of ...
Q: Draw the chair conformation of B-D-Altropyranose given the structure of D- Altrose. (Upload your ans...
A: Chair conformation is the most stable structure of a cyclohexane ring. in the chair conformation, th...
Q: A small peptide has two pKa values of 3.42 and 8.74. What is the isoelectric point for this peptide?...
A: Isoelectric point: The isoelectric point(pI) is the pH at which particular molecule carries no net...
Q: Define triacylglycerol
A: In living organisms, fats and oils are most commonly utilized as energy storage. They are fatty acid...
Q: Sam does not consume alcohol, while his brother Sebastian regularly drinks large quantities of alcoh...
A: Liver, the largest gland in the human body has various secretory and metabolic functions. It secrete...
Q: MATERIALS: Red Cabbage (sliced) PROCEDURE: 1. Wash red cabbage well. 2. Bring the water to a boil. 3...
A: Red cabbage is rich source of pigment called Anthocyanin. It is water soluble compound and depending...
Q: Based on our class discussion of the currently accepted mechanism for ribosome catalysis, draw a gra...
A: By transferring the nascent peptide chain from P-site peptidyl-tRNA to A-site aminoacyl-tRNA (aa-tRN...
Q: A) illustrate in molecular detail how hemoglobin's reduced oxygen affinity is caused by protonation ...
A: The importance of histidine residue in haemoglobin is it stabilizes the the oxygen binding by the hy...
Q: Explain how the carbonate-bicarbonate buffer system works in balancing acid-base in the blood.
A: Buffers are solutions that have weak acid and its conjugate base. They nullify small changes in the...
Q: Speculate on what the receptor sites for each of these molecules might be in terms of shape and pola...
A: Receptors are membrane proteins consisting of proteins, and glycans. Its extracellular domain contai...
In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence of salt and protein?
Step by step
Solved in 5 steps with 1 images
- You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock solution is 5M. a)How would you make 300mL of a 1X solution? b)What is the concentration of NaCl in your 1X solution?Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?What is the minimum length of the column required to completely separate the amino acids? What is the time required for solvent elution (i.e. time for all amino acids to exit the column)?
- To analyze a sample by mass spectrometry, the sample must consist of gas phase ions. a) Explain why it is difficult to make gas phase ions of proteins. b) Generally explain how electrospray ionization turns proteins in solution into gas phase ions.You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?Why is it important to select immiscible solvents when performing liquid-liquid extraction? In general, how can you determine which layer (aqueous or organic) is on top, and which is on the bottom?
- You have a stock solution of 40% hydrogen peroxide. You need to make several dilutions for a calibration curve. Calculate how much volume of the stock hydrogen peroxide you need to make 50 mL of 11% hydrogen peroxide.(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
- Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pHAnswer the following questions about protein concentration determination. a) At what wavelength should an albumin protein solution placed in a quartz cuvette be measured in the spectrophotometer? Why is that? b) With which methods other than spectrophotometer can the amount of protein be measured quantitatively?