Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase. i) Sketch the apparatus set up for the chromatographic technique used by Iman. Use the correct chromatographic terminologies to label all of the apparatus, materials, and phases. ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer. iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.
Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase. i) Sketch the apparatus set up for the chromatographic technique used by Iman. Use the correct chromatographic terminologies to label all of the apparatus, materials, and phases. ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer. iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.
Chapter33: High-performance Liquid Chromatography
Section: Chapter Questions
Problem 33.15QAP
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Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase.
i) Sketch the apparatus set up for the chromatographic technique used by
Iman. Use the correct chromatographic terminologies to label all of the
apparatus, materials, and phases.
ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer.
iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.
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