How might a frame shift mutation in the lacy region influence the phenotype of this bacteria when glucose is abundant in the cell? • How would a missense mutation in lacl alter the amount of B-Galactosidase present in the cell? ● If wild type E. coli were exposed to a growth medium containing glucose, lactose and cyclic adenosine monophosphate, what would happen to the lac operon? Repressor gene lacl Regulatory region Promoter-Operator B-Galactosidase gene P O lacz Structural genes Permease gene lac Operon lacy Transacetylase gene lacA

How might a frame shift mutation in the lacy region influence the phenotype of this bacteria when glucose is abundant in the cell? • How would a missense mutation in lacl alter the amount of B-Galactosidase present in the cell? ● If wild type E. coli were exposed to a growth medium containing glucose, lactose and cyclic adenosine monophosphate, what would happen to the lac operon? Repressor gene lacl Regulatory region Promoter-Operator B-Galactosidase gene P O lacz Structural genes Permease gene lac Operon lacy Transacetylase gene lacA

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

See similar textbooks

Similar questions

Ⓒ Macmillan Learning Classify the given examples of prokaryotic gene expression as positive or negative gene regulation. 54 $ R In the presence of excess tryptophan, a repressor protein binds the operator of the trp operon and prevents the operon from being transcribed. In the absence of lactose, the lacR repressor protein binds the lac operon. F4 Positive gene regulation In the presence of the sugar arabinose, an activator protein binds the promoter of the genes responsible for processing arabinose and induces their transcription. In the presence of iron, the dtxR repressor protein binds DNA, and the gene that encodes for the diphtheria toxin is not expressed. % 5 In the presence of lactose and low glucose, the lac operon expressed 20-fold higher than in the absence of lactose. T F5 < 6 MacBook Air MA F6 Answer Bank & 7 F7 Y U * 8 DII F8 Negative gene regulation 1 ( 9 F9 O ) - C J F10 | ! LIC
In the presence of cephalosphorin, what will be the protein product of the prokaryotic cell gene shown below? 5'-CATGTCAGTAAGGCATCT-3' (Assume this is a segment of a larger structural gene that will be translated into protein in its entirety.)
The previously accepted model of the chloramphenicol action was that it inhibited all ribosomes equally. Why were the authors of the Marks, 2016 paper skeptical of this model? Choose all that are correct. Because they had observed that certain bacteria were resistant to chloramphenicol, and this proves that chloramphenicol stalls ribosomes at certain sites within those bacteria. Because certain MRNA templates had been observed to be inhibited by chloramphenicol more strongly than others Because chloramphenicol induces expression of chloramphenicol resistance proteins through translational arrest at specific codons in the leader ORFS of chloramphenicol resistance genes, which suggests there is preferential stalling at certain sites. Because chloramphenicol induces expression of chloramphenicol resistance proteins - therefore, these proteins must be able to be translated during chloramphenicol treatment. Because chloramphenicol binds the decoding center of the 30S subunit, and there are…
In the absence of cladosporin, explain the initiation steps in the synthesis of lysyl-tRNA synthetase enzyme or protein in the bacterial cell, with the involvement of all initiation factors, each site in the ribosome, any important conserved sequence, subunits of ribosomes, initiation codon, charged tRNA containing what amino acid, whether it requires ATP or GTP
1. Describe which enzymes are required for lactose and tryptophan metabolism inbacteria when lactose and tryptophan, respectively, are (a) present and (b)absent. 2. Contrast positive versus negative regulation of gene expression. Describe therole of the repressor in an inducible system and in a repressible system.
Finish the translation story: - fully explain the G factor stories for each step – and the antibioticthat inhibits it.o how is the next aa brought into the A site of the ribosome and how is it chosen to be thecorrect one. How does streptomycin impact this?o How does the ribosome connect the growing chain of aa to the next one in the A site?How does chloramphenicol impact this?o How does the ribosome step to the next codon? How does spectinomycin impact this?
In 2005, Frederick Blattner and his colleagues foundthat E. coli cells have a global transcriptional programthat helps them forage for better sources of carbon. Many genes, including genes needed for bacterial motility, are turned on in response to poorer carbonsources so that the bacteria can search for better nutrition. You now want to search for genes that regulatethis response. How could you use lacZ fusions to tryto identify such regulatory genes?
Which of the following will result in an INCREASE in the levels (# of molecules) of the beta-galactosidase protein in bacteria? A mutation that disrupts the RNA coding region for lacZ A mutation that disrupts the promoter for the lac repressor (P+) A mutation that dirsrupts the RNA coding region for the lac repressor (lacl) A mutation that disrupts the lac protmoter (PL+) A mutaiton that disrupts the lac operator (OC) 00000
How do sigma factors help regulate gene expression during the transition to stationary phase? O Sigma factors create extra nutrients by fixing CO2 and N2 in the air, thereby preventing the onset of stationary phase. O Different sigma factors recognize different promoters. Sigma factors that recognize stress response and catabolic metabolism promoters are expressed during stationary phase and the housekeeping sigma factor is downregulated, Thus, transcription shifts toward stress response and away from growth, O Sigma factors bind the ribosome and block it from translating certain MRNAS that no longer need to be translated in stationary phase O Signa factors are transcriptional inhibitors, they stop transcription entirely so the bacteria don't waste energy on transcription during starvation O Stationary phase is caused by rifampin treatment, and sigma factors block rifampin activity. thereby preventing the onset of stationary phase.
Provide a detailed description of gene expression and control in prokaryotes. Provide a detailed description of proteins critical for this process. (please hand draw a figure showing gene expression and control in prokaryotes and the proteins involved)
INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.
Read aloud V Draw Highlight 2. You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase). (a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities. (b) You identify another loss-of-function mutation with the following expression pattern: Media + glucose - lactose + glucose +lactose - glucose - lactose - glucose + lactose lacZ expression Low Low High High What components of the lac operon could be mutated to produce this phenotype? List all possibilities.