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- One of the blocking solutions that is traditionally used is non-fat milk, but this is not recommended for studies of phosphoproteins. Why?(MB = methylene blue) What is the concentration of the sample of the substance with the reporter absorbance of 0.40 at 595 nm?What effect does a negative effector have on the graph of reac- tion rate (V) vs. [substrate] for an allosteric effector?
- List three examples of commonly employed combination of ligand and protein in affinity chromatb) Consider the following experimental results: Total hydrolysis of a nonapeptide gave: Ala,Arg, Glu,Leu,Lys,Met,Phe,Tyr,Val One fragment from CNBR treatment was: Tyr-Leu-Lys One fragment from Chymotrypsin treatment was Arg-Glu-Met-Tyr One fragment from Trypsin treatment was Val-Ala-Phe-Arg i. Deduce, with suitable explanations, the amino acid sequence of the nonapeptide.b) Consider the following experimental results: Total hydrolysis of a nonapeptide gave: Ala,Arg,Glu,Leu,Lys,Met,Phe,Tyr, Val One fragment from CNBr treatment was: Tyr-Leu-Lys One fragment from Chymotrypsin treatment was Arg-Glu-Met-Tyr One fragment from Trypsin treatment was Val-Ala-Phe-Arg ii. The reagent shown below is widely utilized in the sequencing of peptides. Treatment of the nonapeptide you deduced in part i. with this reagent yields a phenylthiohydantoin derivative, compound 1, and an octapeptide. Write a mechanism for the formation of compound 1. N=c=8
- If 17 Beta estradiol can diffuse through cell membranes, explain why all cell types are not sensitive to the presence of 17 beta estradiol?Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB? What is the equation for the double-reciprocal plot at 20 nMnM NAMB?Define the term Allosteric Modulation?
- CYP2E1 has a much higher Km for ethanol than ADH (11 mM versus 0.05 mM,respectively). When greater portions of ethanol are ingested (0.08% or 17 mM), which part of the statement would be true?Explain why the negative charges incorporated in the filtering membrane are able to reject negative charge proteins but not chlorine iron.Explain the principles of the redox code. Distinguish between redox switches and redox sensors.