EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Table 1: The initial cell counting before cryopreservation Quadrant Death cell Live cell 1 15 0 3 7 1 5 15 0 7 15 0 9 14 0 Total 66 1 67 Table 2 : Total cells concentration before cryopreservation Total cells 67 Total viable cells 66 Percentage of viable cells (%) 98.51 Average of viable cells 13.2 Cell concentration (cells/ml) 264000 Table 3: Total cells concentration after cryopreservation Total cells 46 Total viable cells 28 Percentage of viable cells (%) 60.87 Average of viable cells 5.6 Cell concentration (cells/ml) 112000 Question: From the table(result) given, please make a discussion The discussion should discuss what is cryopreservation (1), how cryopreservation is useful to make this experiment successful (2), what can be observed from total cells concentration before and after cryopreservation (3), what are the reasons for the changes in total cells concentration after cryopreservation (4)
EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES
Table 1: The initial cell counting before cryopreservation
|
Quadrant |
Death cell |
Live cell |
|
1 |
15 |
0 |
|
3 |
7 |
1 |
|
5 |
15 |
0 |
|
7 |
15 |
0 |
|
9 |
14 |
0 |
|
Total |
66 |
1 |
|
67 |
Table 2 : Total cells concentration before cryopreservation
|
Total cells |
67 |
|
Total viable cells |
66 |
|
Percentage of viable cells (%) |
98.51 |
|
Average of viable cells |
13.2 |
|
Cell concentration (cells/ml) |
264000 |
Table 3: Total cells concentration after cryopreservation
|
Total cells |
46 |
|
Total viable cells |
28 |
|
Percentage of viable cells (%) |
60.87 |
|
Average of viable cells |
5.6 |
|
Cell concentration (cells/ml) |
112000 |
Question:
From the table(result) given, please make a discussion
The discussion should discuss
what is cryopreservation (1),
how cryopreservation is useful to make this experiment successful (2),
what can be observed from total cells concentration before and after cryopreservation (3),
what are the reasons for the changes in total cells concentration after cryopreservation (4)
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