Which among the following statements is correct? O lon-exchange chromatography is dependent on the charge and size of the target component Affinity chromatography is dependent on the affinity of the target component to the mobile phase Both statements are correct Both statements are incorrect
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- Which among the following statements is correct? Negatively-charged proteins are eluted out first when a cation exchanger is used as the stationary phase for ion- exchange chromatography O Proteins that have higher affinity to the ligands are eluted out last in affinity chromatography O Both statements are correct O Both statements bre incorrectWhich among the following statements is correct? Group of answer choices Both statements are incorrect Negatively-charged proteins are eluted out first when a cation exchanger is used as the stationary phase for ion-exchange chromatography Both statements are correct Proteins that have higher affinity to the ligands are eluted out last in affinity chromatographyUsing a semi-permeable membrane, dialysis allows the removal of salt ions prior to chromatography. O True False
- You are designing a medical device to collect Carcinoembryonic Antigen (CEA, MW = 180,000 g mol^-1), a tumor marker, from patients in order to create a personalized cancer vaccine. In this medical device, the cellular components of the blood are removed from the plasma. The plasma then flows into an extraction chamber. Within this chamber, the fluid flow divides into 48,000 10-cm long tubes with an inside diameter of 175 um. The surface of each tube is coated with a material that very rapidly adsorbs CEA. Plasma containing 6.1 ug m1^-1 of CEA flows into the chamber at 125 ml min^-1. Assume that the fluids are at body temperature (37°C). The viscosity and density of water at 37°C are 0.691 cP and 0.993 g/ cm^3, respectively. The diffusivity of CEA inside the extraction chamber is 6.1 x 10^-6 cm^2/s and the mass transfer coefficient for CEA inside the device is: k = 1.25 x 10^-5 cm s^-1. •If you run the extraction for 1 hour and extract 31.7 mg of CEA, what must be the concentration of…You are designing a medical device to collect Carcinoembryonic Antigen (CEA, MW = 180,000 g mol^-1), a tumor marker, from patients in order to create a personalized cancer vaccine. In this medical device, the cellular components of the blood are removed from the plasma. The plasma then flows into an extraction chamber. Within this chamber, the fluid flow divides into 48,000 10-cm long tubes with an inside diameter of 175 um. The surface of each tube is coated with a material that very rapidly adsorbs CEA. Plasma containing 6.1 ug m1^-1 of CEA flows into the chamber at 125 ml min^-1. Assume that the fluids are at body temperature (37°C). The viscosity and density of water at 37°C are 0.691 cP and 0.993 g/ cm^3, respectively. _what is the mass transfer coefficient (cm s^-1) for CEA inside the device ?You are designing a medical device to collect Carcinoembryonic Antigen (CEA, MW = 180,000 g mol^-1), a tumor marker, from patients in order to create a personalized cancer vaccine. In this medical device, the cellular components of the blood are removed from the plasma. The plasma then flows into an extraction chamber. Within this chamber, the fluid flow divides into 48,000 10-cm long tubes with an inside diameter of 175 um. The surface of each tube is coated with a material that very rapidly adsorbs CEA. Plasma containing 6.1 ug m1^-1 of CEA flows into the chamber at 125 ml min^-1. Assume that the fluids are at body temperature (37°C). The viscosity and density of water at 37°C are 0.691 cP and 0.993 g/ cm^3, respectively. A) Estimate the diffusivity of CEA within the extraction chamber?
- In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in thefigure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal fromFilter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose4 and justify your selection.# Filter type WavelengthA Long pass 650 nmB Short pass 530 nmC Long pass 490 nmD Band pass 550-590 nmE Long pass 560 nmF Short pass 640 nmG Long pass 620 nmH Band pass 620-640 nmI Short pass 590 nm(e) Two ADP agonists (drugs) were also found to bind to platelets: 2-methylthio-ADP bound with a Kp of 7 µM and 2-(3-aminopropylthio)-ADP bound with a Kp of 200 µM. Can these drugs effectively compete with ADP for binding to platelets? Explain your answer.Your patient Jane Doe weighs 198lb. You have 1 L bag of saline (from which you have removed 10ml) and a drug pump which can be set to run between 1 and 10 ml/min. She has an order for the drug CONVERTIN to be given at a dose of 300 ug kg hr. A vial of CONVERTIN contains 90mg of the drug in 10ml of saline. After adding the vial of CONVERTIN to the bag of saline and connecting it via pump to the patient's IV line, at what rate should the pump be set in order to deliver the drug dose as ordered?
- The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109 erythrocytes will form a monolayerof area 0.89 m2 when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2 in area.Show that these data can be accounted for only if the erythrocyte membraneis a bilayer.The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109erythrocytes will form a monolayer of area 0.89 m2when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2in area. Show that these data can be accounted for only if the erythrocyte membrane is a bilayer.uring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asap
