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- does TGF-beta treatment cause cells to grow more or less in the soft-agar assay?Will the results of an in vitro antibiotic sensitivity test always agree with the results of an in vivo use of the antibiotic? Why or why not?Regarding the cultivation of animal cells in vitro, it is correct to state that: *(Only one statement is correct) a) Transformed cells differ from normal and established cells in that they have the ability to proliferate indefinitely in culture b) Transformed cells derive from isolated and dissected tumors, constituting cell lines that have limited cultivation time c) The genetic instability presented in normal cells makes them preferential models for the production of recombinant proteins d) Cells cultured in adhesion must be periodically expanded, through the use of enzymes such as trypsin e) The metabolism of carbon sources in the culture medium by animal cells generates products such as lactate, which positively influence their productivity
- Which of the following best explains why there is no growth on plate II? (a) The initial bacterial culture was not ampicillin-resistant. (b) The transformation procedure killed the bacteria. (c) Nutrient agar inhibits bacterial growth. (d) The bacteria on the plate were transformed. (e) The initial bacterial culture is ampicillin-resistant.-Two hundred patients without chest pain were screened with a new biochemical test that showed 190 had a negative screening result. What is the test's specificity? a) 80% b) 95% c) 90% d) 75%What is the reason for using sterilized filter paper in CHORIOALLANTOIC MEMBRANE (CAM) Assay?
- You are asked to design an assay to measure the concentration of WonderDrug in various different pharmaceutical preparations using either its UV-visible absorption or its fluorescence emission. Explain when a UV-visible absorption based assay would be the more appropriate and when a fluorescence based assay would be more appropriate.A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial activity. Live bacterial cells are detected using “Live clear", a dye which is initially coloured blue, but turns clear in the presence of live bacterial cells. The control wells are shown below. Penicillin kills bacteria; glycerol does not affect them. Which of the control wells will appear blue at the end of the assay? "Live clear" glycerol penicillin - - + (A1 A2)(A3)A4) bacterial cells B1 (B2 (B3)B4 А4, В1, B2, ВЗ, B4 A2, A4, B2, B3, B4 А4, B2, ВЗ, В4 A1, АЗ, В1, В2, вЗ, В4 + + |3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?
- What are the advantages and disadvantages of differential centrifugation?You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and notice that the sample from the control cells (not treated with drug) turns purple upon addition of the MTT and the sample from the cells treated your drug are clear after adding MTT. As you walk down the hall to run your sample on the spectrophotometer, how are you feeling? (a) frustrated, (b) unsure, (c) excited. I understand that if the cell are alive, they will have functioning mitochondria, the mitochondria will be producing NADH and NADPH which will allow some enzymes in the cell to convert empty T which is colorless, into a colored form as and die. The colorelss means less dye with present, which means the less empty T was converted, meaning that we had less mitochondrial activity, which means we had less live cells. I think that the answer should be (a) frustrated. I am not sure if I am right. Glad if the expert advise.You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions: a) Which technique will you use to isolate mitochondria? b) What happens to the pH of the medium when the medium is kept anaerobic? c) What happens when O2-saturated saline is added to the mixture?