You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and notice that the sample from the control cells (not treated with drug) turns purple upon addition of the MTT and the sample from the cells treated your drug are clear after adding MTT. As you walk down the hall to run your sample on the spectrophotometer, how are you feeling? (a) frustrated, (b) unsure, (c) excited. I understand that if the cell are alive, they will have functioning mitochondria, the mitochondria will be producing NADH and NADPH which will allow some enzymes in the cell to convert empty T which is colorless, into a colored form as and die. The colorelss means less dye with present, which means the less empty T was converted, meaning that we had less mitochondrial activity, which means we had less live cells. I think that the answer should be (a) frustrated. I am not sure if I am right. Glad if the expert advise.

You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and notice that the sample from the control cells (not treated with drug) turns purple upon addition of the MTT and the sample from the cells treated your drug are clear after adding MTT. As you walk down the hall to run your sample on the spectrophotometer, how are you feeling? (a) frustrated, (b) unsure, (c) excited. I understand that if the cell are alive, they will have functioning mitochondria, the mitochondria will be producing NADH and NADPH which will allow some enzymes in the cell to convert empty T which is colorless, into a colored form as and die. The colorelss means less dye with present, which means the less empty T was converted, meaning that we had less mitochondrial activity, which means we had less live cells. I think that the answer should be (a) frustrated. I am not sure if I am right. Glad if the expert advise.

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter30: The Art Of Image Critique

Section: Chapter Questions

Problem 7RQ

See similar textbooks

Similar questions

Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. 1) What is the importance and advanatge of detecting CSCs? 2) What kind of systems have been developed to detect CSCs? Describe by giving examples. Please explain in detail with your own words.
The diagram shows a simplified cross-section of a human body and an ultrasound transducer.. a) Explain what happens to the ultrasound signal as it travels from the probe through the body. b) Sketch a graph showing how the intensity of the signal detected by the probe varies with time following the transmission of a pulse of ultrasound with the probe in this position
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. 1) What is the importance and advanatge of detecting CSCs? 2) What kind of systems have been developed to detect CSCs? Describe by giving examples.
For this exercise, an electron micrograph of secretory cells of the anterior pituitary is being used (Sandborn, E.B. (1970) Cells and Tissues by Light and Electron Microscopy, New York, New York: Academic Press, Inc.). The image originally measured 18.7 cm wide and 18.5 cm high with a magnification of 5200x. Given the width of the original micrograph at 18.7cm, what is the true width of unmagnified image? Show calculations.
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What kind of systems have been developed to detect CSCs? Describe by giving examples. Please explain with a few examples.
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What kind of systems have been developed to detect CSCs? Describe by giving examples. Please explain in detail the main findings with your own words.
Ms. Michi is a researcher in a renowned pharmaceutical company based in Singapore. One of the cell lines in the lab is suspected to be contaminated. She is culturing NIH 3T3 mouse embryo fibroblast cell line (NIH/3T3 (ATCC® CRL-1658TM). Figure 1 below illustrates her observation under the inverted phase contrast microscope. a) b) c) d) e) Figure 1 (Routray et al., 2016) What type of contamination is her laboratory facing? Explain your answer. Give FOUR (4) suggestions to Michi in order to prevent such contamination in future Besides the contamination stated in Question 2 a), name FIVE (5) other contaminations that Michi should be aware of. During a routine check, Michi's superior requests her to characterize cell lines available in the storage to determine their authenticity. Describe TWO (2) effective cell line characterization methods that she can utilize for this purpose. The pharmaceutical company wants to improve its toxicity testing by replacing monolayer culture with spheroid…
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What kind of systems have been developed to detect CSCs? Describe by giving examples. Thank you.
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs?
In the more common protocol for immunofluorescence detection of cellular proteins, an investigator uses two antibodies. The first binds specifically to the protein of interest. The second is labeled with fluorochromes for easy visualization, and it binds to the first antibody. In principle, one could simply label the first antibody and skip one step. Why use two successive antibodies?
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs? Make a list of it.
A local community is experiencing a surge in lung cancer cases. Residents blame the increase on a chemical plant that opened last year.A number of pollutants emitted from the plant are found to be positive in the Ames Test. What are arguments against this plant being a cause of increased cancer incidences (limit 5-6 sentences)?