Colony of (bacteria): A cluster of cells (or clones) which arise from a single bacterium by asexual reproduction Sample to be counted Dilution 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 9-ml broth 1/10 1/100 (10-') (10 1/10 (10) 1/10 (10) 1/105 (10) 1/10 (10) Plate 1-ml samples When a sample is plated, each colony that grows represents a single cell (or spore) in the original sample 159 17 Too many colonies colonies colonies colonies colonies to count Colony count multiplied by the dilution factor

Colony of (bacteria): A cluster of cells (or clones) which arise from a single bacterium by asexual reproduction Sample to be counted Dilution 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 9-ml broth 1/10 1/100 (10-') (10 1/10 (10) 1/10 (10) 1/105 (10) 1/10 (10) Plate 1-ml samples When a sample is plated, each colony that grows represents a single cell (or spore) in the original sample 159 17 Too many colonies colonies colonies colonies colonies to count Colony count multiplied by the dilution factor

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter15: Antibiotic Agents

Section: Chapter Questions

Problem 19RQ

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Identify A (name of the structures; blank 1) - ldentify B (name of the cells; blank 2) - Identify C (name of the cells; blank 3). High mag Low mag high. mag A Blank # 1 Blank # 2 Blank # 3
1 ml 1 ml 1 ml 1 ml 1 ml Original inoculum 9 ml broth in each tube Dilutions 1:10 1:100 1:1000 1:10,000 1:100,000 1 ml 1 ml 1 ml 1 ml 1 ml Plating 1:100,000 (10-5) 1:10 1:100 1:1000 (10-2) 1:10,000 (104) (10-1) (10-9) 1.Choose a plate that appears to have between 30 and 300 colonies. Count the exact number of colonies on that plate using the colony counter. Colony Count = 64 2. Calculate the number of CFUS per ml of original sample as follows: number of CFUS per ml of sample = (number of colonies) X (dilution factor of the plate counted) = The number of colonies = The dilution factor of plate counted = The number of CFUS per ml
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Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample=number of bacteria/ml (For example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 9 ml broth in each tube 1:10 1:10 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1 ml 1:100 1:1000 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 ml 1:100,000 Calculation: Number of colonies on plate x reciprocal of dilution of sample number of bacteria/ml (For example, if 32 colonies are on a plate of 10.000 dilution, then the count is 32 x 10,000=320,000/ml in sample.) Copyright © 2004 Pearson Education Inc.publishing a Benjenin Cummings
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