An RNA aptamer is a sequence that can fold into a specific three-dimensional structure and bind to a ligand with high affinity. RNA aptamers that bind to specific fibroblast growth factors have shown promise as anticancer drugs, because they can bind and neutralize pro-growth signaling cascades. In the study by Amano et al. the authors developed a collection of RNA sequences that showed optimal binding and specificity for Fibroblast Growth Factor 5 (FGF5). In the table below are some FGF5 binding data for the lead RNA aptamer sequences: Aptamer Koff (x 10-5s-1) 20±6 10+2 2.1 +0.3 12.5± 0.7 "The kinetic parameters are represented by the mean ± standard error from three independent measurements. F5f1 F5f3 Kon (x 105 M-1 S-¹) 3.3 ±0.3 1.7 +0.3 1.8 0.3 1.36 +0.05 F5f1_56 F5f3_56 Kd (nm) 0.7 ± 0.2 0.57 +0.02 0.118 +0.003 0.92 +0.04
An RNA aptamer is a sequence that can fold into a specific three-dimensional structure and bind to a ligand with high affinity. RNA aptamers that bind to specific fibroblast growth factors have shown promise as anticancer drugs, because they can bind and neutralize pro-growth signaling cascades. In the study by Amano et al. the authors developed a collection of RNA sequences that showed optimal binding and specificity for Fibroblast Growth Factor 5 (FGF5). In the table below are some FGF5 binding data for the lead RNA aptamer sequences: Aptamer Koff (x 10-5s-1) 20±6 10+2 2.1 +0.3 12.5± 0.7 "The kinetic parameters are represented by the mean ± standard error from three independent measurements. F5f1 F5f3 Kon (x 105 M-1 S-¹) 3.3 ±0.3 1.7 +0.3 1.8 0.3 1.36 +0.05 F5f1_56 F5f3_56 Kd (nm) 0.7 ± 0.2 0.57 +0.02 0.118 +0.003 0.92 +0.04
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter32: The Reception And Transmission Of Extracellular Information
Section: Chapter Questions
Problem 21P
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