A third spectrophotometric method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal standard. A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2+ yields a ratio of (SA/SIS)std of 2.37. A sample of blood is spiked with the same concentration of Cu2+, giving a signal ratio, (SA/SIS)samp, of 1.80. Determine the concentration of Pb2+ in the sample of blood.

A third spectrophotometric method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal standard. A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2+ yields a ratio of (SA/SIS)std of 2.37. A sample of blood is spiked with the same concentration of Cu2+, giving a signal ratio, (SA/SIS)samp, of 1.80. Determine the concentration of Pb2+ in the sample of blood.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.22QAP: The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of...

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The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of five 50.0-mL volumetric flasks. Various volumes of a standard containing 12.2 ppm Cr were added to the flasks, following which the solutions were diluted to volume. Unknown,mLStandard, mLAbsorbancc 10.00.00.201 10.010.0 0.292 10.020.0 0.378 10.030.0 0.467 10.040.0 0.554 (a) Plot the data using a spreadsheet. (b) Determine an equation for the relationship between absorbance and volume of standard. (c) Calculate the statistics for the least-squares relationship in (b). (d) I)ctcrmine the conccnt ration oÍCr in ppm in the sample. (e) Find the standard deviation of the result in (d).
Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC system to a mass spectrometer is a much more difficult task. Describe the major reasons why it is more difficult to combine HPLC with mass spectrometry than it is to combine GC with mass spectrometry.
A spectrophotometric method for the quantitative determination of Pb2+ levels in blood uses Cu²+ as an internal standard.A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2* yields a ratio of SA/Sis of 2.37.A sample of blood is spiked with the same concentration of Cu2* giving a signal ratio of I.80. Determine the concentration of Pb2+ in the sample of blood.
A spectrophotometric method for the quantitative analysis of Fe2+ in blood uses Zn2+ as an internal standard. A sample containing Fe2+ and Zn2+ with final concentration of 3.25 ppb and 0.75 ppb, respectively, that yields a signal of 0.423 for Fe2+ and 0.650 for Zn2+. Subsequently, a sample of blood is spiked with Zn2+ to give the same final concentration and yielded a signal of 0.832 for Zn2+ and 0.110 for the unknown concentration of Fe2+. Determine the concentration of Fe2+ in the sample of blood.
A spectrophotometer method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal standard. A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2+ yields a signal of 0.832 for Pb2+ and a signal of 0.351 for Cu2+. A sample of blood is spiked with the same concentration of Cu2+, giving a signal of 0.520 for Pb2+ and 0.289 for Cu2+. Determine the concentration of Pb2+ in the sample of blood.
A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.
6. In a flame spectrometric determination of sodium, lithium is used as an internal standard. Assume the spectrum of a standard solution that contains 2.00 ppm Na and 100 ppm Li shows two peaks with intensities of Ina= 4.22 and I= 9.24, respectively. An unknown sample solution that also contains 100 ppm Li gives two peaks with intensities of Ina = 6.16 and I= 8.24, respectively. Calculate the concentration of Na in the unknown sample in ppm. А. 3.08 рpm В. 6.16 рpm С. 4.22 рpm D. 3.27 ppm Е. 12.1 ppm
6. In a flame spectrometric determination of sodium, lithium is used as an internal standard. Assume the spectrum of a standard solution that contains 2.00 ppm Na and 100 ppm Li shows two peaks with intensities of Ina= 4.22 and IL= 9.24, respectively. An unknown sample solution that also contains 100 ppm Li gives two peaks with intensities of Ina = 6.16 and I= 8.24, respectively. Calculate the concentration of Na in the unknown sample in ppm. А. 3.08 ppm В. 6.16 рpm C. 4.22 ppm D. 3.27 ppm Е. 12.1 ppm
A spectrophotometric method was developed to determine the Pb2+ in blood using Cu2+ as an internal standard. A standard 1.75 ppb of Pb2+ and 2.25 ppb of Cu2+ gives a signal ration of 2.37. A blood sample, to which the same amount of internal standard was added, gave a signal ration of 1.80. Calculate the Pb2+ in the blood sample. A. 1.33 ppb B. 13.3 ppb C. 1.75 ppb D. 7.47 ppb
Inductively coupled plasma–optical emission spectrometry (ICP-OES) was used for the determination of cadmium using indium as an internal standard. A solution containing 2.15 ppm Cd2+ and 5.76 ppm In3+ gave signals of 19,291 and 37,190 counts for cadmium and indium, respectively. A 0.01652 g sample was dissolved in acid and quantitatively transferred to a 250.0 mL volumetric flask. A 1.00 mL aliquot of 264.5 ppm In3+ was added and the flask was diluted to volume with deionized water. ICP-OES analysis of the sample solution gave signals of 31,109 and 118,941 counts for cadmium and indium, respectively. What was concentration of the Cd2+ in the sample? Give answer all questions with explanation
Internal standard. Mn was used as an internal standard for measuring Fe by atomic absorption. A standard mixture containing 2.00 g Mn/mL and 2.50 g Fe/mL gave a quotient (Fe signal/Mn signal) 1.05. A mixture with a volume of 6.00 mL was prepared by mixing 5.00 mL of unknown Fe solution with 1.00 mL containing 13.5 g Mn/mL. The absorbance of this mixture at the Mn wavelength was 0.128, and the absorbance at the Fe wavelength was 0.185. Find the molarity of the unknown Fe solution.
In spectrophotometric analysis, the relationship between the absorbance A and the analyte's concentration C is: A = ebC + Ablank What is the molar extinction coefficient I and its absolute uncertainty if A is 0.68 ± 0.06; Ablank is 0.06 ± 0.01; b is 1.00 cm; and C is (0.45 ± 0.03)x10-5 mol/L?