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- ExplaintheadditionalhealthriskstoapatientundergoingaCT scan as compared to an ordinary X-ray photograph.When observing relatively thicker slices of tissue or specimens,.A. you may need to refocus slightly to see cells or structures that may be deeper ormore superficialB. you should focus on the most superficial layers of cellsC. you should focus on the deepest layers of cellsD. you should focus as closely to the middle of the specimen as possiblea. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.
- 2. Below are images showing the cell envelopes of Gram-positive and Gram-negative bacteria. Indicate which one is Gram positive and which one is Gram negative. Following Gram staining, what would be the color associated with each type of cell? Also write the names of the structures to which the lines are pointing. (A) Gram color of cells after Gram stain: (1) (2). (3) 50 nm Moobiskyy AnEverimy Kience Third EHilien Figure 3.15.02 SCOURTESY OF BENOIT ZUBER/MRC. O ORYOF MOLECULAR BIOLOIn order to visualize the fine structure of viruses and cytoskeletal filaments at 10-25 nanometers in diameter the type of microscopy that would be most effective is O standard light microscopy O phase-contrast light microscopy transmission electron microscopy O darkfield light microscopy O differential-interference microscopyYou are scheduled to give an intramuscular injection in theventrogluteal site and remember learning that it should beadministered using the Z-track technique. When you mentionthis to the nurse in the hospital who has been taking care ofyour patient, she tells you there is no reason to use the Z-tracktechnique. You remember your instructor telling you to use theZ-track technique. What should you do?
- The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1). Identify B (name of the structures arrows point to; blank 2) - Identify C (name of the structures arrows point to; blank 3) High mag low mag high mag Blank # 1 Blank # 2 Blank # 3Which of the following is an advantage of confocal microscopy over conventional fluorescence microscopy? The interaction of the laser beam with the cell surface allows the imaging of individual macromolecules O Only out-of-focus light enters the microscope lens, making the object appear illuminated against a dark background The use of electrons instead of light to image the specimen results in greatly increased resolving power O Optical sections in a single plane of focus can be visualized at different depths in the specimen The natural contrast of an unstained specimen is enhanceda. Explain the purposes of tissue fixation and tissue staining. b. Identify the factors that affect the quality, of tissue fixation and describe the effects of fixation on tissue (e.g., Temperature, size of tissue, time of fixation, or osmolality of fixative.) c. Describe the following types of microscopes and describe reasons for their use. a. Light (compound microscopy), b Polarizing microscopy, c. Phase-contrast, d. Interference microscopy, e. Darkfield, f. Fluorescence, g. Electron (scanning and transmission). h. Confocal microscope. d.…
- Recall from the video the steps of how to make a wet mount of cheek epithelial cells. Place the steps of how to prepare a wet mount in the correct order from left • View Available Hint(s) Reset Help Add a drop of physiological saline to the center of the slide. Scrape the inside of the cheek with a toothpick. Stir the end of the toothpick in the physiological saline. Mix a drop of stain into the specimen preparation with a toothpick. Place a coverslip on top of the specimen.What type of microscopy is frequently used now for determining the cellular location of a molecule of interest? Briefly describe the basis of this technique.ARTEFACTS Tissue is usually fixed and embedded in such material as paraffin, gelatine, resin etc. prior to sectioning. A range of knives (metal, glass, diamond) are used on various cutting machines -microtomes to obtain the sections. As a result, the tissue can sometimes be pushed out of shape and scratches, tears, or folds can appear. Excessive drying can create spaces that do not exist, and uneven thíckness can cause unusual colouring effects. These extra features are called artefacts. Virtual Slides Description of Artefact Kidney , human Small intestine,(jejunum), human Aorta, human Trachea, human Tendon, cat Thyroid gland, human Gall bladder, human