ARTEFACTS Tissuo is usually fixed and embedded in such material as paraffin, gelatine, resin etc. prior to sectioning. A range of knives (metal, glass, diamond) are used on various cutting machines -microtomes to obtain the sections. As a result, the tissue can sometimes be pushed out of shape and scratches, tears, or folds can appear. Excessive drying can create spaces that do not exist, and uneven thickness can cause unusual colouring effects. These extra features are called artefacts. Virtual Slides Description of Artefact Kidney , human Small intestine,(jejunum), human Aorta, human Trachea, human Tendon, cat Thyroid gland, human Gall bladder, human
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
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