A sample of Melbourne drinking water was injected into an ion chromatograph. The anions were separated isocratically via ion-exchange using 4 mM NAOH as eluent and detected by conductivity after chemical suppression. The system void volume (t) occurred at 1.25 minutes, while chloride and sulfate were eluted at 3.12 and 6.62 minutes respectively. The eluent concentration was then increased to 10 mM The new retention time for chloride is:
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- percentage purity of an ibuprofen sample was determined by a reversed phase HPLC method as follows: Y Der ● A calibration curve of peak area versus concentration (ug/mL) of pure ibuprofen was constructed The ibuprofen sample for analysis was prepared by taking 10.0204 g of powder and dissolving it in/1Lbf distilled water (solution A). 1 ml of the latter solution was further diluted to IL with distilled water (solution B). Solution B was analysed by HPLC analysis Results The equation for the best-fit line of the calibration curve is: y=26820x-1323.8. ● The average peak area for the ibuprofen sample (solution B) was 266796.2 Calculate the percentage purity of the ibuprofen powder.A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μm4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
- A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc, was found to be 209.6 s with a baseline peak width, w., of 14.4 s. The retention time for aspartame, ta, was 260.0 s with a baseline peak width, wa, of 21.2 s. The retention time for the unretained solvent methanol was 49.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = um %3D Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2. R2N =The data in the following table were extracted from a gas chromatographic analysis of a two- component mixture. Air nonane decane Retention time (s) 5 26 32 Peak width, baseline (s) 5 4 (i) Calculate the capacity factor, k, for the two compounds. (ii) Calculate the selectivity factor, a, for the two compounds.A solution of volume 1 mL, containing an equal parts mixture (by weight) of aspirin and paracetamol (acetaminophen), was analysed by HPLC, displaying peaks at retention times 1.5 mins (area integration 1500) and 2.0 mins (area integration 2500). An unknown solution, also volume 1 mL, was analysed under the same conditions and, among a number of other peaks, there was one at 2.0 mins (area integration 4000). (i) How can the 2.0 min peak in the unknown sample be confirmed as paracetamol? (ii) In a subsequent analysis, 5.0 mg of aspirin was dissolved in the unknown solution and an extra peak appeared in the HPLC at 1.5 mins (area integration 2000). Assuming the 2.0 min peak confirmed as paracetamol, determine its concentration (g L-¹) in the unknown sample and estimate the error in the determination.
- Injection of ethane, propane, butane, pentane, and hexane on a squalene column under isothermal conditions gives retention times of 3.51, 4.98, 7.30, 11.00, and 16.85, respectively. Air, which is nonretained, elutes at 1.00 min. An injection of pyridine under the same conditions gives a retention time of 10.05 min. What is the Kováts retention index for pyridine under these conditions? O A. 334 O B. 300 O C. 485 O D. 478 O E. 370A 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to 100.0 mL in a volumetric flask. Aliquots of the sample solution were treated with a lead complexing reagent and water as follows: Solution 1: 10.0 ml blood sample + 20.0 mL complexing agent + 30.0 mL H20. Solution 2: 10.0 ml blood sample + 20.0 mL complexing agent + 26.0 mL H20 + 4.00 mL of 78 ppb Pb2+ standard. The resulting solutions were analyzed by UV/Vis at 375 nm. Absorbance for solution 1 = 0.155 and for solution 2 = 0.216. Calculate the concentration of lead (ppb) in the original sample.1. Calculate the difference in retention volumes for solutes A and B that are passed through a column containing 1.8 mL of stationary phase and 2.7 mL of mobile phase. The distribution coefficients of A and B are 8.5 and 16.8 respetcively.
- The percentage purity of a paracetamol sample was determined by a reversed phase HPLC method as follows: • A calibration curve of peak area versus concentration (ug/mL) of pure paracetamol was constructed The paracetamol sample for analysis was prepared by taking 10.0254 g of powder and dissolving it in IL of distilled water (solution A). 1 mL of the latter solution was further diluted to 1L with distilled water (solution B). Solution B was analysed by HPLC analysis Results • The equation for the best-fit line of the calibration curve is: y = 26811x – 1313.8 The average peak area for the paracetamol sample (solution B) was 266796.2 Calculate the percentage purity of the paracetamol powder.Researchers used gas chromatography to determine the % v/v methyl salicylate in rubbing alcohol [Van Atta, R. E.; Van Atta, R. L. J. Chem. Educ. 1980, 57, 230-231]. A set of standard additions was prepared by transferring 20.00 mL of rubbing alcohol to separate 25 - mL volumetric flasks and pipeting 0.00 mL, 0.20 mL, and 0.50 mL of methyl salicylate to the flasks. All three flasks were diluted to volume using isopropanol. Analysis of the three samples gave peak heights for methyl salicylate of 57.00 mm, 88.5 mm, and 132.5 mm, respectively. Determine the % v/v methyl salicylate in the rubbing alcohol.A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, 0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. c) Calculate the Rf values if the solvent front is 18 cm.
