A direct current was passed through a solution containing valine histidine and aspartic acid at a pH of 6.0 .One amino acid migrated to the poritive electrode ,one migrated to negative electrode and one did not migrate to either electrode .Which amino acids went where?

Q: At pH 7.4, the pH of blood plasma, do the majority of protein-derived amino acids bear a net…

A: Concept introduction: Isoelectric point (pI) is defined as pH at which the protein has no net…

Q: In a solution at pH 7.0, a polymer of lysine( polylysine) is a random coil with no secondary…

A: Polylysine forms a random coil at low pH , alpha helix at a pH above 10.6 and gets transformed into…

Q: At a steady state in the living cell, (ATP) = 10-3M and (ADP) = 10-4 M. The maximum (equilibrium)…

A: First with the value of K, the overall reaction is: Glycerol + ATP ----------> Glycerol…

Q: lonization State of Histidine Each ionizable group of an amino acid can exist in one of two states,…

A: “Since there are multiple questions and it is not mentioned that which one has to be solved so I am…

Q: amino acid will elute first

A: For cation exchange column, pH is kept slightly lower in comparison to pI value so that the…

Q: Glutamic acid has three ionizable groups. The pKa of a-COOH is 2.19; a-NH 3+, 9.67; and the R-group,…

A: Given - Pka of α-COOH of glutamic acid = 2.19            PKa of α-NH3+ of glutamic acid = 9.67…

Q: How would you make 2.0 L of electrotransfer buffer for transferring the proteins from a gel onto…

A: The composition of electrotransfer buffer is given as 1) Concentration of Tris (this is a solid; MW…

Q: Hemoglobin A has a pI value of 5.6 whereas the variant hemoglobin M has an aspartate residue in…

A:

Q: True or False An essential amino acid is one which is not synthesized in the body and thus must be…

A: 1.) An essential amino acid is one which is not synthesized in the body and thus must be supplied in…

Q: At what pH will the amino group of cysteine be 50% deprotonated? The pKa values are 2.2, 8 and 9.6?…

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Q: Draw the different ionization structures and identify the isoelectric pH of the following amino…

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Q: Draw the structures of the amino acids phenylalanine and aspartate in the ionization state you would…

A: We have been asked to draw the structures of phenylalanine and aspartate at pH = 7 We have been…

Q: 3) Calculate the physiological AG for the reaction: Phosphocreatine + ADP → creatine + ATP at 25 °C…

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Q: Reaction of the a-carboxyl group of an amino acid or peptide with phenylisothiocyanate allows for…

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Q: The concentration of a biomolecule inside a rod-shaped prokaryotic cell is 0.0043M. Calcuate the…

A: Solution stoichiometry involves the calculation of concentration of solutions in the given…

Q: The initial pH of a 2M arginine solution is 14. What is the predominant structure of arginine in…

A: At pH = 14 , arginine exists in completely deprotonated form .

Q: Show the shift of the electron density and the appearance of partial charges on carbon atoms in the…

A: Given : To determine the electron density and the appearance of partial charges on carbon atoms in…

Q: The amino acid His is triprotic with pK1 (α-COOH) = 1.82, pK2 (α-NH3+) = 9.17, and pK3 (side chain)…

A: If the pKa of acid is equal or lower than the pH of the solution then the acid will get deprotonated…

Q: Calculate the pH at which the e-amino group of lysine is 25% dissociated.

A: Step : 1             Calculate the pH at which the e-amino group of lysine is 25% dissociated.

Q: Which of the following tetrapeptides will be retained on a cation-exchange column at pH 8.2? O HGTH…

A: Ion exchange chromatography separates ions and molecules based on their net overall surface charge.…

Q: Calculate the number of millimoles(mmol)in 500 mg of each of the following amino acids: alanine…

A: The relation between mass, molar mass and mole is given by : Mole = mass/molar mass

Q: In basic medium which of the following amino acids migrate slower than others towards positive…

A: 1. Amino acids are chemical compounds, made by the attachment of -COOH and -NH2 groups. 2. According…

Q: Give the numbers of peptide bond present in given structure, structure is given at pH 10.5,  find…

A: The CO- NH bond is called peptide bond. A peptide bond connects two Amino acid units. Amino acid…

Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…

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Q: me amino group = 9.04, and pKaR for the side chain - 12.48

A: Deprotection of groups depends upon the it's pKa value when pH > it's pKa then deprotection…

Q: Which of the following amino acid's side chains will become protonated and carry a +1 charge when…

A: Basic amono acids                                                               Pka values…

Q: The isoelectric point (pl) of a peptide is the pH at which the Group pK peptide ceases to migrate in…

A:

Q: Two phenylalanine residues are separated by 1.0 nm in a polypeptide. Calculate the dispersion…

A: Dispersion force is the force of attraction that exist between atoms or molecules that developed…

Q: what is pseudo order treatment

A: Pseudo is Latin word which means ‘fake’. A Pseudo order reaction can be defined as a bimolecular…

Q: The state of ionization of a specific amino acid is directly influenced by the pH of the surrounding…

A: The state of ionization depends upon the pH of  the medium. Lysine is a basic amino acid having two…

Q: Plot the titration curves of valine and lysine amino acids in basic medium and calculate the pI…

A: amino acids have ionisable group which ionizes at different condition depending on pH of the…

Q: Calculate the volume, in liters, of 1.666 M KOH that must be added to a 0.129 L solution containing…

A: The given data contains, mass of glutamic acid hydrochloride = 9.42 g. Molar mass of glutamic acid…

Q: Given the following peptideSEPIMAPVEYPK(a) Estimate the net charge at pH 7 and at pH 12. Assume the…

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Q: "A protein has a pI point which can be used to separate proteins using ion exchange. If you have an…

A: PI value or isoelectric point of a protein is its pH at which it carries no net electrical charge…

Q: Draw the structure of the tripeptide Tyr-Gly-Lys as it exists at pH 13. and what if it was at an…

A:

Q: Determine the buffer capacity of Alanine and Histidine at pH 2.0 and 6.0 respectively and discuss…

A: For the alanine, the value for pKa is 2.34. Now the salt to amino acid ratio to achieve the pH of…

Q: Calculate the pH at which the e-amino group of lysine is 25% dissociated. Calculate the pH at which…

A: In order to find the pH at which the ε-amino group of the lysine dissociates we first write done the…

Q: An amino acid residue side chain is deprontonated at pH9.5. What is the pKa of this residue if it is…

A: an animo acid is a biomolecule which as both basic amine group and acidic group ( carboxylic acid)…

Q: Ornithine is an amino acid that is not used in the synthesis of proteins, but is an important…

A: orthinine has a 3 ionizable groups. 1. amino group 2. carboxylic group 3. and a side chain pka of…

Q: A theoretically computed equilibrium constant for the polymerization of formaldehyde, HCHO to…

A: Given data contains, Molarity of glucose solution is 1.00M.

Q: The amino acid histidine has a side chain for which the pKa 6.0. Calculate what fraction of the…

A: from Henderson Hasselbach equation we have pH = pka + log [conjugate base] /[ acid form] acid form…

Q: For the amino acid alanine, the major species in solution at pH 7 is the zwitterionic form, which…

A: Here, we have to estimate the ratio of the concentration of the neutral amino acid species to the…

Q: Calculate AG for the creation of an electrochemical gradient of protons in a mitochondrion, when the…

A: Given that, R=8.314 J/mol.K,      T=298K    , F=96485 C/mol     and pH=7and 8 ∆G=RHTHlnxinxout,Here…

Q: All amino acids have two ionizable functional groups: an α‑amino group (average pKa of 9.4) and an…

A: The question is based on the concept of amino acids. we have to identify at which pH, this structure…

Q: Draw the lewis structures of the following: alanine, asparagine, cysteine, glutamine, glycine,…

A: Nitrogen-containing amino acids are essential for life and they are the building blocks of protein.…

Q: A compound is known to have a free amino group with a pKa of 8.8, and one other ionizable group with…

A: Given compound is having two ionisable groups.

Q: Which amino acid has the greatest amount of negative charge at pH = 6.20?

A: The has two group over it the acid group and they carry both charges depending on the pH of both the…

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.