4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH-8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form?
4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH-8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form?
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 7.7P
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Need help with question 4, thank you
Transcribed Image Text:4:47 PM Wed Mar 27
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5 of 8
primer 3'-end
WAT1
2.12
H₂O
dCTP
2.25 (2.86)
2.11
(N/A) -
2.25
(2.44)
MgA
2.35 (2.45)
(2.26)
2.01
(2.31)
3.79
MgB
1.87'
1.86 (3.87)
1.98 (2.50)
(2.41)
(2.39)
1.86
1.97
1.87
(2.24)
(2.45)
(2.34)
WAT2
D654
A476
D475
4. RNA hydrolysis
As discussed in class, we can predict how much of RNA we expect to be in a reactive form using
general chemistry calculations(Henderson Hasselbach).
a) Calculate the percent of RNA at pH=8 that will have the 3'OH in its reactive alkoxide
form (O-). The pKa of the 3'OH is approximately 13.
b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10.
c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA
to be in its reactive form?
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