3. A linear DNA fragment is cleaved with one, two or three restriction enzymes to yield fragments as follows: Enzyme(s) HindIII Smal Fragments (kb) 2.5, 5.0 2.0, 5.5 HindIII+ Smal 2.5, 3.0, 2.0 HindIII+ Smal+ EcoRI 1.5, 2.0, 2.5 Draw the restriction map of the original DNA fragment, indicating the positions of all restriction sites.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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3. A linear DNA fragment is cleaved with one, two or three restriction enzymes to
yield fragments as follows:
Enzyme(s)
Fragments (kb)
HindIII
2.5, 5.0
Smal
2.0, 5.5
HindIII + Smal
2.5, 3.0, 2.0
HindIII+ Smal+ EcoRI 1.5, 2.0, 2.5
Draw the restriction map of the original DNA fragment, indicating the positions of all
restriction sites.
Transcribed Image Text:3. A linear DNA fragment is cleaved with one, two or three restriction enzymes to yield fragments as follows: Enzyme(s) Fragments (kb) HindIII 2.5, 5.0 Smal 2.0, 5.5 HindIII + Smal 2.5, 3.0, 2.0 HindIII+ Smal+ EcoRI 1.5, 2.0, 2.5 Draw the restriction map of the original DNA fragment, indicating the positions of all restriction sites.
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