In chain termination sequencing, which of the following terminates the chain? a. The lack of a base at the 1' carbon b. The lack of OH at the 2' carbon c. The lack of OH at the 3' carbon d. The lack of phosphate at the 5' carbon
Q: Because of the metastatic trait of cancer cells, how can we judge a cancer treatment to be…
A: Cancer symptoms can be treated with radiation and chemotherapy, among other treatments. Other drugs…
Q: Describe the whole process of photosynthesis.
A: Introduction :- Plants and other living things use a process called photosynthesis to transform…
Q: Nitrogen bases pair with bases that are _____. a. Available b. Identical c. Complimentary d.…
A: DNA is the genetic material in living organisms that is made up of nucleotides. Nucleotides are…
Q: and Lamarck's theory of evolution and of course the differences between both theories
A: Lamarck and Darwin both were evolutionists that gave their theory of evolution. Some of the points…
Q: Label the structures in the following illustration of a synovial joint. (a). (d) C d (b)
A: Introduction The area of the body where two or more bones come together to allow for movement is…
Q: Ⓒ Sally A. Morgan/Ecoscene/CORBIS According to the Costa Rica Rainforests Outward Bound School, the…
A: Introduction Rainforest comprises tall, evergreen tree with high rain fall. It is also called the…
Q: which statement about capillaries is true? a Capillaries carry blood away from the heart. b…
A: The branch of science that deals with the study of blood vessels is known as angiology. The blood…
Q: 7. Which cytokines are involved in regulating the acute inflammatory response?
A: Introduction: Small secreted proteins called cytokines that are released by cells have a particular…
Q: Explain some molecules are at equilibrium across a biological membrane and yet their concentration…
A: Introduction Biological membranes are made up of two sheets of lipid molecules (known as a bilayer).…
Q: I. The Mendelian pattern of inheritance is a general term that refers to any pattern of inheritance…
A:
Q: "To judge the biological importance of an interaction between protein A and protein B, we need to…
A: Physical interactions between two or more protein molecules that have a higher degree of specificity…
Q: Why is it not 1.5 or 3 or 6? 180/60=3 to give you 3/2=1.5
A: DHFR is an enzyme that is take part in pyrimidine biosynthesis pathway. It is responsible for the…
Q: Question 24 If the cell has a mutant nonfunctional CDC25, where does it arrest? O O G2 G1 M
A: A cell has four distinct phases in the cell cycle; which are called Interphase (G1), synthesis or S…
Q: A chiasma (plural = chiasmata) is (check all that apply): a physical connection between linked genes…
A: The examination of a human or another organism's entire DNA (containing all of its genes). An…
Q: You are studying the M-cyclin. You treat mitotic cells with an inhibitor of the proteasome and find…
A: Cyclins are a family of proteins that bind to CDKs to activate them even if they do not themselves…
Q: magine that you created a cell that over-expresses a truncated version of its extrinsic pathway…
A: The outcome would have no effect on extrinsic apoptosis.
Q: CREATE a workout plan to improve your swimming (Freestyle and Backstroke)
A: *Workout plan to improve swimming *Freestyle swimming workout: *A good…
Q: Which of the following methods has the least ability to sterilize below the surface? In other words,…
A: Disinfection is killing or removing pathogenic microbes and is mainly used for nonliving things.…
Q: What’s a nucleotide
A: Introduction :- Nucleotides are organic molecules made up of a phosphate and a nucleoside. They…
Q: How many carbon atoms exit every Calvin cycle process? 0 1 3 6 12 18
A: In Calvin cycle there are three basic steps that is carbon fixation, reduction and regeneration.…
Q: . When performing a bacterial lab, what could happen that could lead to incorrect results (potential…
A: While performing a bacterial lab, a lot of precautions have to take in order to reduce potential…
Q: Bacteria between the gums and tooth that causes inflammation there is called: 1)dental caries 2)root…
A: Answer and explanation - Gingivitis-Gingivitis is mild gum disease. It is an infection caused by…
Q: Plz answer these 2 question Importance of diet as a contributing factor to gout Guidelines…
A: Gout can be defined as a type of arthritis in which the level of uric acid in the blood increases…
Q: Which of the following is not a post-translational modification? Select an answer and submit. For…
A: Please follow step 2 for detailed explanation.
Q: D. A null mutation in the smarty gene results in no functional SMARTY protein produced. nottulum…
A: DISCLAIMER FOR MULTIPART Since you have posted a question with multiple sub-parts, we will solve…
Q: Muscle hypertrophy training is catalyzed by which of the following? Mechanical tension, high reps,…
A: Introduction: Muscle hypertrophy happens when net protein balance is favourable over time and muscle…
Q: Write explanatory notes on the morphology and importance to man of the following high G + C Gram…
A: Introduction Small, single-celled organisms are known as bacteria. Nearly every place on Earth…
Q: A drug inhibits positive regulation of an operon. What is the most likely outcome? a. No mRNA…
A:
Q: Why is Arabidopsis thaliana a good model organism ? Why is it essential for GUS ?
A: Arabidopsis thaliana is a small flowering plant. it is an angiosperm, a dicote from mustard family.…
Q: What is another name for a scientific test or a set of tests
A: Scientific method is a way of discovering knowledge about a natural phenomenon based on predictions,…
Q: Examine whether the statement "Like the lumen of the ER, the interior of the nucleus is…
A: Introduction: The endoplasmic reticulum and the nucleus are membrane-bound organelles that are…
Q: The primary function of chaperones is to ... Select an answer and submit. For keyboard navigation,…
A: Chaperones are the proteins that facilitate the proper folding of the proteins. These proteins are…
Q: 2.1 In your own view, discuss the possible route of transmission of SARS-CoV-2 from an animal source…
A: "Virology" is a field of microbiology that studies many forms of viruses, covering their features,…
Q: Did you mean 0.000034 instead of 0.000024
A: NADPH Nicotinamide adenine dinucleotide phosphate abbreviated NADP+ or NADPH is an essential…
Q: If grapes are the most common food source for most of the years, and drought occurs infrequently,…
A: Introduction : Darwin used the phrase "survival of the fittest" to describe how natural selection…
Q: How the low pH of lysosomes protect the rest of the cell from lysosomal enzymes in case the lysosome…
A: Protein molecules known as enzymes speed up processes without altering the course of the process. As…
Q: The binding of methotrexate interferes with mitochondrial import of the enzyme DHFR which was…
A: As part of the de novo manufacture of purines, an enzyme called DHFR (DiHydroFolate Reductase)…
Q: Explain what would happen to a substrate molecule if it encountered an enzyme’s active site that…
A: Enzymes are biocatalyst that perform specific chemical reactions in the living organisms. They are…
Q: According to the cladogram shown, which organisms are most closely related? Highlight or circle your…
A: Introduction Phylogenetics is a strong method for determining the evolution of modern species.…
Q: Explain the use of passing the inside-out vesicles contaminated with right-side-out vesicles through…
A: Vesicles are usually defined as the way that snipped from the trans-Golgi in the introduction. The…
Q: Please explain your choice: Consider an individual with genotype A/a B/b C/c D/d, descended from one…
A: Genetic linkage is the tendency of genes to be inherited together during meiosis. The closer the…
Q: Do the comparison between Darwin's and Lamarck's discovery of evolution, by mentioning what are the…
A: Evolution is the accumulation of genetic variations. It results in the formation of new structures…
Q: explain in which cycle of PCR is a double-stranded fragment of correct size first generated.
A: A DNA segment can be amplified in a single copy or a few copies using the polymerase chain reaction…
Q: Describe the structure of a chloroplast, and specify the location of each process of photosynthesis.
A: Introduction :- Since photosynthesis occurs in chloroplasts, which are organelles in plant cells…
Q: When protein kinase A (PKA) is inactive, which of the following statements is true? ATP is bound to…
A: Protein kinase A (PKA) is a family of enzymes whose activity is dependent on cellular levels of…
Q: How and when is carbon dioxide reduced during photosynthesis? Please be specific.
A: Plants and other living things employ a process called photosynthesis to transform chemical energy…
Q: The nephron filters 180L of filtrate and condenses it to 1.5-2L of urine True False
A: Introduction :- Your kidneys are each made up of a million nephrons, which are filtering cells. Each…
Q: "Transporters saturate at high concentrations of the transported molecule when all their binding…
A: Specialized membrane transport proteins known as transporters and channels are used by cells to…
Q: type of mutations would be the worst types
A: Mutation: It is defined as the change in the DNA base pair sequence due to the environmental…
Q: List down the factors that can cause contamination to your culture. 2. Why does the pH calibration…
A: there are various factors that can contaminate of our culture by in contacting with the culture…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Sanger sequencing (aka the chain termination) a. is a method for for determining the sequence of nucleotides in a piece of DNA of intrest. b. requires use of special ddNTPs (dideoxy nucleotides) that have special florecent lables (dyes) attached to them and also lack a 3' hydroxyl. c. requires fragments to be run through a tube containing a gel matrix where their fluorescent dyes are illuminated with a laser, which can be used to produce a chromatogram. d. all of the above are true e. none of the above are trueWhat is the effect of incubating a mixture of RNA and DNA with 0.1 M NaOH? A. DNAishydrolyzedtonucleosidemonophosphates.B. No effect. RNA and DNA are resistant to base hydrolysis.C. RNA is hydrolyzed to nucleoside monophosphates. D. Both RNA and DNA are hydrolyzed to nucleoside monophosphates. Stem-loop structures in RNA resemble the ________ form of double-helical DNA. A. A B. BC. supercoiledcircular D. ZConsider the following segment of DNA:5′ GCTTCCCAA 3′3′ CGAAGGGTT 5′Assume that the top strand is the template strand usedby RNA polymerase.a. Draw the RNA transcribed.b. Label its 5′ and 3′ ends.c. Draw the corresponding amino acid chain.d. Label its amino and carboxyl ends.Repeat parts a through d, assuming the bottom strand tobe the template strand.
- Answer the following parts: A. When performing classical Sanger or "dideoxy" sequencing, you set up 4 parallel reactions per template to be sequenced from a specific primer, with each of the four reactions containing a different dideoxynucleotide, and then the four reactions were run in a separate, adjacent lanes on a gel. Why couldn't you combine all 4 dideoxynucleotides with the primer and the template and do the whole reaction in one tube, and then run the set of fragments produced by the reaction mixture on a single lane in an acrylamide gel? B. When doing automated sequencing, on the other hand, all 4 dideoxynucleotides are added to the same sequencing reaction, and run together in a single capillary gel. What's the difference - why can an automated sequencing reaction be done with all 4 dideoxynucleotides mixed together and all the resulting fragments run together, but not a conventional sequencing reaction?Please list all possible products in a sequencing reaction using ddGTP as a terminator based on the following sequence of a template. 3’ – GGGCTATACCTGACCCTC – 5’Consider the following DNA strand with the following nucleotide sequence: 3’-ATATCAGAGAATATCA-5’ The nucleotide sequence of the complementary DNA strand is . b. The nucleotide sequence of the antisense strand used in the transcription process is . c. The nucleotide sequence of the mRNA strand produced after the transcription process is 2. Compute for the base composition of a DNA molecule given that %T is 23% (reported to the nearest whole number; no need to add the % symbol). % A? %C? %G?
- Procedure This activity will use the Human β-hemoglobin gene, which is mutated in sickle cell anemia, with the following sequences of the first thirty (30) nucleotides: TAC CAC GTG GAC TGA GGA CAC CTC TTC AGA... 1. First transcribe the DNA sequence into the mRNA sequence. 2. Refer to the genetic code to write down the amino acid sequence that these 30 nucleotides encode beginning with the first nucleotide. 3. Generate a random number (1-30) by drawing lots in a bowl. Then locate the DNA nucleotide to "mutate" using the number drawn as the position along the gene. 4. "Roll" the tetrahedron "mutator" dice (see direction below for making the tetrahedron "mutator" dice). Note the letter on the side that is flat on the table. That is the nucleotide that will replace the nucleotide in the DNA at the position decided in the previous step. 5. Write the mutant nucleotide sequence in the row for Mutation 1, then analyze mutation. a. If it is the same nucleotide, write same nucleotide…If you repeat the okazaki experiment WITHOUT denaturing DNA, what would be the expected outcome? a. Increased length of RNA primers b. Slowing of fragment maturation O c. No short fragments observed d. All short fragments observed e. Decrease in fragment size over timeWhich of the following is true regarding next generation sequencing? 1. Next generation sequencing is also known as sequencing by synthesis 2. Next generation sequences uses DDNTPS to identify the base at the end of the nucleotide chain 3. Next generation sequencing will not work if chain terminating nuclear tides are included in the reaction 4. Next generation sequences uses fluorescently labeled ddNTPs instead of radioactivity labeled ddNTPs 5. Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time
- Which of the following sequences would most likely be able to bind a Cyclic AMP DNA binding protein? (only one strand is shown but assume DNA is double stranded) A. 5' ATAGTOCATAGTC 3' B. 5' ATCGGTCTCGAT 3' C. 5' GCTCTAGCGAGC 3' D. 5' ATAGTAATGATA 3'Design a strategy to make a subtracted-complete cDNA library from liver cells. Use a flowchart method to propose how this objective can be accomplished. The sizes of mRNA range from 2 kb to 15 kb. Briefly indicate your reasons for the sequence of steps..Given is the 30 nucleotides in the human gene for hemoglobin (the oxygen-carrying protein in the red blood cells): 5’ TAC-CAC-GTG-GAC-TGA-GGA-CTC-CTC-TTC-AGA 3’ a. What is the complementary strand? b.Deduce the mRNA in this coding region. c.What is the amino acid sequence based on this mRNA? d. A very important mutation in human hemoglobin occurs in this DNA sequence, where the T at nucleotide 20 is replace with an A. The mutant hemoglobin is called sickle cell hemoglobin and is associated with severe anemia. What is the amino acid replacement that results in sickle-cell hemoglobin?