1a. suggest a test you will use to show that a given food substance contains protein. b. show how you will use i. modified Gabriels synthesis ii. Streckers synthesis to prepare phenylalanine in the laboratory.
Coding Strand of DNA
When pointing to DNA transcription, the coding strand is found to be the DNA strand whose base sequence is indistinguishable from the base sequence of the RNA transcript developed. It is this strand that comprises the codons, while the non-coding strand comprises the anti-codons.
Nucleotide
Both DNA and RNA are composed of organic molecules known as nucleotides. Hence, nucleotides are known as the basic building blocks of nucleic acids. These substances play a role in various processes such as cell signalling, enzyme reactions, metabolism, and so on.
Structure of Cytosine
Cytosine is among the five primary nitrogenous bases of which DNA and RNA and are being used in storage and transportation of genetic makeup within a cell. Adenine, guanine, thymine as well as uracil are the remaining four nucleobases.
1a. suggest a test you will use to show that a given food substance contains protein.
b. show how you will use
i. modified Gabriels synthesis
ii. Streckers synthesis to prepare phenylalanine in the laboratory.
2a. Describe in details how you will determine the primary structure of protein.
b. You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino acids are as follows,
Histidine 7.64
Lysine 9.74
Cysteine 5.02
Show how you will separate the mixture into the pure forms. State and describe any instrument that you will use to separate the components.
3a. A decapeptide has the following amino acid composition,
Ala2, Arg, Cys, Glu, Gly, Leu, Lys, Phe, Val
Partial hydrolysis yields the following tripeptides,
Cys-Glu-Leu +Gly-Arg-Ala +Lys-Val-Phe-Gly
Reaction of the decapeptide with 2,4-dinitrofluorobenzene yields 2,4-dinitrophenylsine.
From the experimental data, deduce the primary structure of the decapeptide.
b. suggest a scheme you willfollow to synthesize the dipeptide Ala-Gly
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