12. This detector is not used in HPLC: a) ECD b) MS c) Fluorescence d) RI

Q: What cause the spikes in the spectrophotometer reading? Explain

A: In electronic spectroscopy, generally the electron excites  from an initial low energy state to a…

Q: Why is it more difficult to optimize k' and α in HPLC than in GC hand

A:

Q: The obtained plot in the detector of HPLC is called, O peak O bell O triangle area

A: HPLC stands for high performance liquid chromatography which is a separation technique and the…

Q: What is the Rf value for the uppermost yellow spot in TLC plate 3 (plate imaged below)? It is…

A: The TLC plate 3 with all the spots is given below :

Q: The following results were obtained from an analysis of copper content in four representative…

A: Absolute error = Half of the smallest possible measurement Relative error = Absolute error / Actual…

Q: Which is better HPLC system Agilent 1290 Infinity II LC or Nexera Series UHPLC? Explain

A: HPLC is High Performance Liquid Chromatography UHPLC is Ultra High Performance Liquid Chromatography

Q: 3. a. Briefly explain why internal standardization method is useful in analytical chemistry?

A: A)  Internal standards are widely used in chromatography because of differences in the…

Q: PART B. Spectrophotometrilc Determination of the Copper Content of a Penny Mass of penny:3.138 Date…

A:

Q: A student measures the solvent height on a TLC plate as 11.5 cm. Spot A was measured at a height of…

A: Formula used :-  RF = distance traveled by compound/distance traveled by solvent

Q: A) Given the following set of data, plot the chromatogram (absorbance versus volume of eluate). Vol…

A: To plot the chromatogram, the volume of eluate is taken as x axis and absorbance as y axis The graph…

Q: Relative Standard Deviation of UV Vis, please explain.

A: A question based on RSD, which is to be accomplished.

Q: A spectrophotometer method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal…

A: given : SASIS = 0.8320.351= 2.37 CA = concentration of analyte = 1.75 CIS = concentration of…

Q: In spectophotometry, what will happen to the %T if the concentration is doubled?

A: Spectrophotometry is a branch of electromagnetic spectroscopy concerned with the measurement of the…

Q: Calculate the Absorbance from concentration for each trial of 0,10, 30, 40, 50 % (with yellow LED)…

A: The Absorbance from concentration for each trial of 0,10, 30, 40, 50 % has to be calculated.

Q: Mass spectral measurements were made on 10 replicate low concentration Ni-EDTA samples: 171.7,…

A: TRANSPORT-PROPERTY AND MASS SPECTRAL MEASUREMENTSIN THE PLASMA EXHAUST PLUME OF AHALL-EFFECT SPACE…

Q: Which of the following is NOT a possible cause of error when taking a spectrophotometer reading? An…

A: Spectrophotometer is used to measure  absorption if light or the amount of chemicals in a solution.…

Q: Which of the following is not a type of HPLC detector? Select one: O a. Electrochemical O b.…

A: Mixture components are estimated using HPLC detectors. The actual separation in HPLC (High…

Q: 10. which of the followings is the universal detector used in IHPLC: a) Fluorescence b) IR c) FID d)…

A: HPLC ("High-Performance Liquid Column") is utilized to separate and distinguish the components that…

Q: Which of the following is correct? Select the correct response: HPLC columns are packed with…

A: Hplc

Q: calculate the following for each sample gas chromatograph. a) retention time for each peak (label…

A: Gas chromatography is a technique for separating and analyzing compounds that can be vaporized…

Q: 10 9. 8 7 6. 4 3. 1 0 ppm Chemical shift (8)

A: The given information is: Chemical formula of the compound = C8H9Br 4 chemical shifts are present:…

Q: 5. Using the diagram answer the questions below: Increasing time Sample appled to top of column…

A: To answer question related to chromatography:

Q: List the desirable characteristics of HPLC detectors.

A:

Q: 2 1.2 1.1 3 0.9 4 0.7 For each set, determine the mean, spread, stand rariation. Calculate the…

A:

Q: 1. Provide two advantages and two disadvantage of flow cytometry analysis. Briefly explain each.

A:

Q: A 10.00 g sample containing an analyte was transferred to a 250 mL volumetric flask and diluted to…

A: Given, sample is 10 g which is transferred to 250 ml volumetric flask.  The first signal after…

Q: What is the application of chromatography (HPLC) and it's mechanism ?

A: Answer.

Q: Compare the quantities For a normal phase chromatographic technique: KA > KB Choose A if Polarity of…

A: Given,               For a normal phase chromatographic  technique :…

Q: What four values are needed to calibrate a CO2 sensor (IRGA)? B. Briefly describe how to obtain…

A: These sensors depends on the IR light source and detects the quantity of CO2 molecules. Calibration…

Q: Reagent blanks are primarily used to: a. align the optics to achieve maximum irradiance. b.…

A: Different types of spectroscopy can be used to identify the structure of unknown compound. Some…

Q: Detector response is corrected to take in account dilution before being plotted on the y-axis. O…

A: True

Q: 5. Draw and label a box diagram for a double-beam spectrophotometer. What advantages does a double…

A: diagram :

Q: A HPLC column had a sample (0.10ml) loaded onto it. The detector volume showed 0.15 mL. From this…

A: Before starting any troubleshooting, whether it is related to instruments or columns, it isessential…

Q: which of the followings is the universal detector used in HPLC: a) Fluorescence b) IR c) FID d) ECD…

A: The universal detector used in HPLC has to be given.

Q: For the data below, Calculate the Minimum Detection Limit based of the Linear Calibration Curve.…

A:

Q: pectrofluorometry is potentially more sensitive and selective compared to spectrophotometry

A:

Q: 4. Calculate the resolution from the chromatogram and table below for: a. peak #2 and peak # 3 b.…

A: To calculate resolution from the given chromatogram: a. peak #2 and peak #3 b. peak #2 and peak #5

Q: If the selectivity factor is equal to 1, the peaks are separated. True or False?

A: The given statement is If the selectivity factor is equal to 1, the peaks are separated.

Q: Mass spectral measurements were made on 10 replicate low concentration Ni-EDTA samples: 171.7,…

A: TOF spectra induced by the fs laser ionization of ethane have been recorded as a function of the…

Q: Does CI aroms have an isotopic pattern of 1:1 relative intensity of M and M+2. True or False?

A:

Q: improve

A: We know that resolution is a measure of how well two Elution peak can be differentiated in…

Q: Mass spectral measurements were made on 10 replicate low concentration Pb-EDTA samples: 152.7,…

A:

Q: 6. The calibration equation obtained by spectroscopic method for chromium (Cr) determination is:…

A: Solution- The average signal to noise (S/N) =26.71/0.08 = 333.875 ~ 334

Q: Comparison of fingerprint region between literature and experimental spectra.

A:

Q: Does the amount of radiation (cpm) increase or decrease as the distance between the sorce and the…

A: Amount of radiation between the source and sensor is determine using the inverse square formula:…

Q: ti n d ĉ Table 2. Concentration and Spectrophotometric data for Brilliant prepared in trials # 1 -…

A:

Q: High performance liquid chromatography (HPLC) can be used to : a. identify the various pigments from…

A: High performance liquid chromatography (HPLC) is defined as a column chromatography where at high…

Q: UVVIS spectrophotometry

A: Correct: A

Q: The basic spectrophotometer is composed of four components. Which component listed below is NOT one…

A: A question based on tools in analytical chemistry that is to be accomplished.