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John Lee Lab Report

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Who Killed John Lee? A Biology 111 Forensic Investigation By: Lauren Mendoza Lab Group: Liz Sanchez and Estelle Makobi Introduction The purpose of this lab is to find out who is the culprit of the murder of John Lee. The victim was found in the CCA Lab Prep room on Monday morning. There were no fingerprints found, but fiber evidence and biological evidence were collected at the scene. The reported time of death was 10:00pm Sunday night. We conducted a series of experiments to find the guilty suspect. There was a total of five suspects. Suspect one is Brandy Smith, John’s current girlfriend, she did have a strong motive to kill John; He had gone out with his ex and Brandy was very mad. Brandy’s alibi included her being at the movie theater …show more content…

In John’s text messages, Brandy texted she would “kill him”. Brandy does have a car and she also has a pet cat. Moving on, suspect two was Sergio Jones, a CCA student and the victim’s lab partner. Sergio had no clear motive to murder John, he was at the library until 8:00pm that night. Sergio does not have a car, but he does have a dog. Next is suspect 3 named Bill Apple, a CCA student and the victim’s lab partner. Bill and the victim had got into a violent argument, Bill claims the two solved the problem and they were supposed to study at the Aurora Central Library the night of the murder and the victim never showed up. Bill has car and both a cat and a dog. Suspect 4, Ann Smith, is a janitor at CCA. Ann did speak with the victim that night, she did say there was others in the building that night, but she cannot identify any one of them. She has no clear motive, she also has a cat. Lastly is suspect 5, Amanda Martinez. Amanda is the victim ‘s ex-girlfriend. John had told her that they would never …show more content…

We were using a restrictive enzyme to cut the DNA into smaller fragments. For the restriction digest we pipet 4 micrometers of enzyme mix into the bottom of each of our colored tubes making sure to use a new tip for each sample. Next, we capped the tubes and mixed the contents by flicking the tube a little bit with our fingers. After we mixed the contents, we tapped the tube to make sure all the liquid would go to the bottom of the tube. Then, we put the colored tubes in the heating blocks and we let them incubate for 35 minutes at 37 degrees Celsius. After the incubation, we removed the tubes from the heating blocks. We then got the package of 1.2% agarose FlashGel. The gel contained a chemical which allows us to see the DNA fragments under the ultra violet light. We marked where each DNA fragment would go on the gel and began to put them in the gel wells. We carefully put 5 micrometers of the crime scene samples into the wells. We had DNA from suspect 1, 2, 3, and 5. We also had the victim’s DNA and two unknown samples found at the scene. When adding the samples to the wells we used a new tip for each sample. We also made sure to wear gloves during this whole process for two reasons. First reason, the chemical used in the FlashGel is toxic and the second reason is to make sure our DNA doesn’t affect the

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