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Name: _____________________________ Date: ______________________________ Bacteria Lab Worksheet Part One: Gram Staining Analysis Review the Step 1 Pre-Lab section on Gram staining and shapes of bacteria to assist you in answering the questions.
1. Observe the micrograph above containing two different bacteria on one slide. The two bacteria are Escherichia coli (E. Coli) and Staphylococcus Aureus (S. Aureus). Remember that Gram-negative are pink or red in color and gram-positive are purple or violet in color. After completing the Gram staining process, the E. Coli is determined to be Gram-negative while the S. Aureus is Gram-positive. Answer the following questions: a. What is the shape of the S. Aureus? What is the shape of the E. Coli? (3 pts)
b. What color is the E.Coli? What color is the S. Aureus? (3 pts)
c. Which bacteria absorbed the counterstain (safranin)? Why? Explain in at least 3 well-written sentences. (6 pts) d. Which bacteria is more resistant to antibiotics? Why? Explain in at least 3 well-written sentences. (6 pts)
2. Gram-Positive Bacteria Analysis a. What carbohydrate comprises 90% of the cell wall? (3 pts)
3. Gram-Negative Bacteria Analysis a. In the extra outer membrane, lipopolysaccharides are considered to be endotoxins. What does this mean? (4 pts)
Part Two: Antibiotic Sensitivity Analysis Review the Step 1 Pre-Lab section on Antibiotic Sensitivity to assist you in answering the questions.
1. Observe the Petri dish above containing the bacteria Staphylococcus aureus (S. aureus). Answer the following questions based on the results from the Kirby-Bauer test. a. Rank the antibiotics from 1-5 starting with the most effective (1) to least effective (5) against the S. aureus in the chart below: (10 pts)
1. 2. 3. 4. 5.
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Related Questions
66671/take/questions/800382
Regarding Gram staining, what will be the appearance of the Gram-negative bacteria
if...
All steps are done correctly?
[Choose ]
[Choose ]
Pink
The slide is not heat-fixed prior to Purple
staining?
Clear
Orange
Crystal violet was omitted?
lodine was omitted?
Only iodine was used?
Acetone:alcohol was omitted?
Too much acetone:alcohol was
used or was left on for too long?
Not enough acetone:alcohol was
used?
Carbol fuschin was omitted?
Carbol fuschin or safranin was
omitted?
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
arrow_forward
Answer True or False
Dr. Soler would like to view the internal structure of the bacteria he was working on. He should use the transmission electron microscope
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MICROBIOLOGY: Microscopic Morphology of Microbes
Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content).
another:
What is the advantage of the Gram stain over the simple stain?
What is the theory about the mechanism of the Gram-stain reaction?
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Question:-
Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need).
Introduction
Discussion
References
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Using a Light Microscope to Determine an Object's SIZE
PRE-LAB QUESTIONS
Fill in the diagram of the microscope with the term or description that matches, the microscope
part.
Eye Plece
Body Tube
Contains lens to increase
magnification usually 10x
Revolves to allow changing
various objectives
Arm
Objectives
Moves stage up and down
approximately to correct
distance
Hold slides in place
Stage
Permits finer focusing by
moving the stage in
smaller increments
Regulates the amount of
light going through the stage
Base
Light Source
Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.
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Indicate the correct way of inoculating the following media.
Plate Media
For sensitivity testing
________________________________________________
For isolation of colonies
___________________________________________________
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Copy and paste the link below and watch the video on Youtube
https://www.youtube.com/watch?v=8RBs0Ghg_48
Answer the following Questions:
1. What are the chemicals and materials used in gel electrophoresis?
2. Draw a schematic diagram of a gel electrophoresis set-up
3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?
4. What is the use of ethidium bromide and why must you wear gloves when you handle it?
5. What makes the DNA fragment move towards the positive plate?
6. What is the purpose of glycerol in the sample buffer?
7. What is the use of a DNA ladder?
8. What will happen when you increase the voltage of the set-up?
9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
arrow_forward
0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red
blood cells if she accidently received an intravenous fluid in the hospital that was made with 0.09%
salt solution
Edit View Insert Format Tools Table
12pt v
Paragraph v
B
I U AV e T?v
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This is a typical lab exam question. (Problem-solving, uses current topic.)Your TA performed the antibiotic disc experiment using supplies from their research lab. The antibiotic discs had the year 2002 written on their containers.Your TA grew a pure culture of unknown bacteria. They applied the culture to a fresh agar plate and applied the 2002 antibiotic discs to the plate and incubated it for growth. They found that the mystery bacteria species was resistant to all three antibiotic discs that were applied to the plate.Are there any control groups that could be used to confirm or refute this multi-drug resistance? Describe them.
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Make a concept map/flow chart for this technique (Cellulose Tape Perianal Swab)
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In order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no
I need help answering this queshtion the answer is with the article and it has to be as short as possible
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
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topic: gel electrophoresis
. What are other staining methods that can be used for PAGE?
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Discuss the steps from dissection of tissue to producing a section on a slide ready for staining, as provided for this practical and as would be carried out in a hospital lab. include principles and procedures for each step. maximum word limit 400
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PROCEDURE:
1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep
things easy.
2. Stick a long piece of tape over the
two pencils and to the table on either
side of the pencils to hold the tape
tightly between the two pencils like
a bridge.
3. Don’t touch the sticky side of the
tape or you will ruin the microscope.
Drop a small drop of water onto the
top of the tape using the pipette or
medicine dropper.
4. Make 3-4 lines of tape and add a
different-sized drop to each one.
This will help determine what size
of water droplet produces the biggest
magnification.
5. Prepare a rectangular shape of plastic cover. Put a small slice of onion.
Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size
of the onion on different droplets. Write your observations on the table below.
No. of drops
Observation
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List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage.
a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage.
4. Use the Low Power Objective.
a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
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Which lab technique can you use to best answer these questions?
How movable are membrane proteins?
[ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP
Where in the cell does your favorite protein localize?
[ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP
How fast do ion channels open and close
[ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP
How big is your protein or DNA
[ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP…
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Answer the worksheet about the bacteria in test material.
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* Question Completion Status:
QUESTION 67
Specimens viewed through the microscope appear:
O reversed (upside down) and inverted (backwards),
O smaller and inverted (upside down).
O inverted (bpside down).
smaller.
O reversed (backwards).
QUESTION 68
What are the sample holders used in the spectrophotometer called?
O Cuvettes
O Volumetric flasks
Syringes
O Pipettes
O Graduated cylinders
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Give the uses/functions and images of each apparatuses.
Basic Laboratory Equipment
Uses/Functions
Picture
1. Microscope
2. Colony Counter
3. Autoclave
4. Microbiology incubator
5. Drying oven
arrow_forward
izzes/00071/take/questions/600381
Regarding Gram staining, what will be the appearance of the Gram-positive bacteria
if...
All steps are done correctly?
The slide is not heat-fixed prior to
staining?
Crystal violet was omitted?
lodine was omitted?
Only iodine was used?
Acetone:alcohol was omitted?
Too much acetone:alcohol was
used or was left on for too long?
I
Not enough acetone:alcohol was
used?
Carbol fuschin or safranin was
omitted?
W
[Choose ]
[Choose ]
Purple
Clear
Pink
Orange
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
[Choose ]
88
22°C
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Direction: Read and analyze the following laboratory experiment and answer the
following question.
PART 1: SURFACE AREA AND CELL SIZE
Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein
cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels.
Methodology:
1. Safety: Wear goggles and nitrile gloves while completing this lab.
2. Obtain three different size blocks of pink or blue agar. Using a ruler,
measure the length, width, and height of the three blocks given below. Cut
the agar according to the given dimension.
Small = 1 cm x 1 cm x 1 cm
Medium = 2 cm x 2 cm x 2 cm
•
• Large = 1 cm x 1 cm x 6 cm
3. Record your data.
4.
Pour HCl or vinegar into two small cups. Place the one larger "cell" into one
cup and the two smaller cells in the other cup. Start timing 30 minutes.
5. After 30 minutes, remove the cells and blot them dry with a paper towel.
6. Using your ruler, measure the distance the HCl has diffused into the blocks
as shown on the…
arrow_forward
Give the uses/functions and images of each apparatuses.
Basic Laboratory Equipment
Uses/Functions
Picture
1. Microscope
2. Colony Counter
3. Autoclave
4. Microbiology incubator
5. Drying oven
6. Refrigerator (microbiology)
7. Bunsen burner/alcohol lamp
8. Candle jar
9. Anaerobic jar
10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet
11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)
12. Petri dish
13. Culture tubes
14. Hanging drop slide
15. Durham’s tube
16. Staining rack
17. Thermostatically controlled water bath
18. Inoculating loop
19. Inoculating needle
20. Vials
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Match the terms on the left with its description
_____ stage
_____ocular lens
____Diaphragm
_____adjustment focusing knob
_____Body tube
holds the microscope slide
lens you look through, usually 10x power
regulate the amount of light passing the specimen
used to focus on specimen
holds the eyepiece
upper part of the microscope that contains optical components.
holds the object lens
magnifies specimen
secures that slice to the stage
condenses light from the light source into a narrow beam
source of illumination
magnifies an image 100x,
arrow_forward
Can someone answer and explain the following?
What are the basic components of a Fluorescence Microscope and what are the functions of each?
Are there any parts that you can remove without compromising accuracy and utility of the equipment?
Can you suggest additional components to improve the equipment?
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I BIO-103-LAB-WORKSHEET-3_BACTERIAL_MORPHOLOGY.docx - Trio Office Writer
File Edit View Insert Format Styles
Table Form Tools Window Help
Abç
#
a a a ab ah
& a
三、三军三E
Default Style
Calibri
12
I 1 I II
Guide Questions:
Illustrate the different forms of bacteria.
1) Spherical - coccus
diplococcus
streptococcus
staphylococcus
mono coccus
2) Rod-shaped bacillus
club-shaped bacilli
rodswith square ends
fusiform bacilli
rods with rounded ends
3) Curved rod - spir illa
a. rigid form
Spirillum
vibrio
Page 2 of 3
143 words, 1,416 characters
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English (USA)
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80%
8:26 AM
P Type here to search
13
15
2/6/2021
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Help with
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I’m kinda confused on what to study for the test and what questions are gonna be on there but this PowerPoint but I just want you to explain the microbiology screenshots to me in a way I can clearly understand, I need a tutor
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M. luteus
~0.8 micron
Arrangement in tetrad
(arrows) and irregular
cluster
10 um
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“Belinda!” called Kyle, running to catch up to her. Belinda turned around and waited for him.
“Are you heading to the lab right now?” asked Kyle.
“Yes.” she said. “I want to look over the Gram stain protocol one more time before we go in.”
“Yeah, good idea. I’ve been trying to memorize the steps. But it’s hard.”
Belinda and Kyle spent the twenty minutes remaining before the start of lab going over Chapters 3 and 4 of their textbook, which listed the steps of the Gram stain and how the staining results relate to the structure of the bacterial cell wall.
Kyle and Belinda took a sample of the bacteria provided by their instructor and each performed the Gram stain. They focused on their slides with the oil immersion lens.
“Got it!” they said almost simultaneously, grinning proudly.
“So, what do you have?” asked Belinda.
“I have Gram-positive bacilli” announced Kyle with an air of triumph.
“Huh? I have Gram-negative bacilli.”
“That’s not right, we used the same sample!…
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Question:-
Your medical care team determines that a third patient's severe illness is caused bya helminth. Which technique or techniques described above would you use to identifythe helminth? Justify your answer.
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Label the key components of a matrix-assisted laser desorption/ionization time-of-flight, or MALDI-TOF, mass spectrometer.
ion source
matrix
laser
Answer Bank
detector
transient recorder
Beam splitter
Trigger
flight tube
O
protein sample
arrow_forward
SEE MORE QUESTIONS
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Related Questions
- 66671/take/questions/800382 Regarding Gram staining, what will be the appearance of the Gram-negative bacteria if... All steps are done correctly? [Choose ] [Choose ] Pink The slide is not heat-fixed prior to Purple staining? Clear Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin was omitted? Carbol fuschin or safranin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]arrow_forwardAnswer True or False Dr. Soler would like to view the internal structure of the bacteria he was working on. He should use the transmission electron microscopearrow_forwardMICROBIOLOGY: Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). another: What is the advantage of the Gram stain over the simple stain? What is the theory about the mechanism of the Gram-stain reaction?arrow_forward
- Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion Referencesarrow_forwardUsing a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.arrow_forwardIndicate the correct way of inoculating the following media. Plate Media For sensitivity testing ________________________________________________ For isolation of colonies ___________________________________________________arrow_forward
- Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?arrow_forward0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red blood cells if she accidently received an intravenous fluid in the hospital that was made with 0.09% salt solution Edit View Insert Format Tools Table 12pt v Paragraph v B I U AV e T?varrow_forwardThis is a typical lab exam question. (Problem-solving, uses current topic.)Your TA performed the antibiotic disc experiment using supplies from their research lab. The antibiotic discs had the year 2002 written on their containers.Your TA grew a pure culture of unknown bacteria. They applied the culture to a fresh agar plate and applied the 2002 antibiotic discs to the plate and incubated it for growth. They found that the mystery bacteria species was resistant to all three antibiotic discs that were applied to the plate.Are there any control groups that could be used to confirm or refute this multi-drug resistance? Describe them.arrow_forward
- Make a concept map/flow chart for this technique (Cellulose Tape Perianal Swab)arrow_forwardIn order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no I need help answering this queshtion the answer is with the article and it has to be as short as possible URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/arrow_forwardtopic: gel electrophoresis . What are other staining methods that can be used for PAGE?arrow_forward
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SEE MORE QUESTIONS
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Recommended textbooks for you
- Case Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage