Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate?

Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate?

Case Studies In Health Information Management

3rd Edition

ISBN:9781337676908

Author:SCHNERING

Publisher:SCHNERING

Chapter3: Informatics, Analytics, And Data use

Section: Chapter Questions

Problem 3.1.1C

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Copy and paste the link below and watch the video in Youtube and Answer the Questionshttps://www.youtube.com/watch?v=8RBs0Ghg_48 Gel electrophoresis Questions 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
This is a paper centrifuge: (you don't need to watch the whole video, just a few seconds to see how it works--description below is probably sufficient if you can't get to the link) https://www.youtube.com/watch?v=isMYGtCFljc It is a paper disc that has supercoiled strings through two holes in the centre--like a button. As you straighten the strings and uncoil them, the paper spins. The paper spins at a very high rate and can separate materials in a way that is similar to a centrifuge. A droplet is added to the centre of the paper and it is spun. The densest objects travel the farthest from the centre. Assume that we are using it to perform our mitochondrial extraction, which cellular component would travel the furthest? nuclei mitochondria microvessicles blood cells
Discuss about Fluorescencemicroscopy
Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
Which lab technique can you use to best answer these questions? How movable are membrane proteins? [ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP Where in the cell does your favorite protein localize? [ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP How fast do ion channels open and close [ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP How big is your protein or DNA [ Choose ] Gel electrophoresis Electron microscopy Fluorescent microscopy Patch clamp PCR FRAP…
וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…
blackboardcdn.com/5bfc08ba3fldc/14683296?X-Blackboard-Expiration=16245468000008X-Blackboard- 19 / 47 100% 1.4. Functions of the light mlcroscope parts Complete the following table by writing the function(s) of each of the parts indicated. Structure Function Diaphragm / iris Stage opehing Lamp Objective lenses Eye piece Coarse and find adjustment knobs Stage Stage rack prt sc delete home backspace lock enter pause t shift
+ courses/_518299_1/cl/outline?customClassicLocation=%2Fwebapps%2FBb-McGraw Hill-BB5744b9beb8ccb%2Fapp%2Flink%2Finbou.... File Edit Format Tools Help Qa 11 Chapter 2 ● Exam 2 Review Sheet ● Normal ● ● ● Unsaved Chantor 7 Edits will not be automatically saved. Calibri T Save now 14 ▾ BIU A A- EEEE VE What are the important characteristics of atoms and elements? What are the different types of chemical bonds? What are the various types of chemical reactions and solutions? What determines acidity and alkalinity, and how do they relate to the pH scale? What makes carbon the fundamental element of life? What are the four types of biological macromolecules, and what are their general structures and functions?
Draw the Haworth projection of B-L-altrose. You will find helpful information in the ALEKS Data resource. Explanation 2 Check # 3 Click and drag to start drawing a structure. $ 4 % 5 80 6 :: & 7 X S * 00 to v © 2023 McGraw Hill LLC. All Rights Reserved. Terr ( 9
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Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?
0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red blood cells if she accidently received an intravenous fluid in the hospital that was made with 0.09% salt solution Edit View Insert Format Tools Table 12pt v Paragraph v B I U AV e T?v