You wish to quantify your favourite compound in an experimental solution usingspectrophotometry. First, you use standard solutions of your favourite compound tocollect the following standard curve data. Calculate the slope of the line of best fitto two decimal places, and enter it in the box below the table.Standard curve dataConc (MM)0.3000.6000.9001.2001.500Abs0.2250.4500.6750.9001.125Next, you prepare an experimental sample by mixing 0.25 mL of the compoundsolution with 9.75 mL of distilled water. Calculate the dilution factor and enter it as awhole number (no decimals).Next, you read an absorbance of 0.72 absorbance units for the diluted sample.Calculate the concentration (mm) of the original solution of the compound. Roundthis answer to one decimal place.Previous PageNext PageMacBook AirPage 5 of 13

Spectrophotometry is a technique to measure the light absorption by a substance. It is classified…

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Understanding Health Insurance: A Guide to Billing and Reimbursement

14th Edition

ISBN:9781337679480

Author:GREEN

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Chapter12: Commercial Insurance

Section: Chapter Questions

Problem 4R

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* You can keep extra decimal places through most of this problem, but round your final estimate to one decimal place* You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit. Standard curve data Conc (mM) 0.300 0.600 0.900 1.200 1.500 Abs 0.150 0.300 0.450 0.600 0.750 Next, you prepare an experimental sample by mixing 0.50 mL of the compound solution with 9.50 mL of distilled water, and you read an absorbance of 0.625 for this sample. Calculate the concentration (mM) of the original solution of compound. Round this answer to one decimal place.
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Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.
Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
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