You are supplied with the following: / Jy word voorsien van die volgende: NaCl (Mr= 58,443 g /mol) 2.5M Tris-Cl, pH 8 solution /oplossing (1 Litre) EDTA (Mr= 380,2g/mol) 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O) You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 7.8P
icon
Related questions
Question

You are supplied with the following: / Jy word voorsien van die volgende:

  • NaCl (Mr= 58,443 g /mol)
  • 2.5M Tris-Cl, pH 8 solution /oplossing (1 Litre)
  • EDTA (Mr= 380,2g/mol)
  • 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing
  • Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O)


You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan:

  • 15m M NaCl
  • 75 mM Tris-Cl,pH 8
  • 16 mM EDTA,pH 8
  • 0.8% sodium sulphate / natrium dodecyl sulfaat
  • 0,75 mg/ml proteïnase K


How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up. / Hoe sal jy 500 ml van die verteringsbuffer voorberei? Wys al jou stappe en berekeninge. Onthou om te verduidelik presies hoe jy dit sal op maak.

Expert Solution
steps

Step by step

Solved in 3 steps with 15 images

Blurred answer
Knowledge Booster
Scientific notation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:
9781305143531
Author:
CURREN
Publisher:
Cengage
Essentials of Pharmacology for Health Professions
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage