Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique
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Q: cold method of Acid-fast staining.
A:
Write the instructions, reagents, principles and the procedures for the following.
1. Periodic acid Schiff technique
2. Grocott's hexamine silver technique
3. Ziehl-Neelsen stain
4. Lactophenol blue stain
5. Mehtamine silver technique
6. Shikata's orcein method
7. Mann's methyl blue-eosin stain
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Solved in 3 steps
- write the protocol for mann's methyl blue-eosin staining, including their reagents, materials, prinicple, procedures results, and cautionsComplete the table below. For the function, identify the reagent if it is a stain, clearing agent, mountant, fixative, dehydrant, embedding medium, or adhesive. Then give 2 common health hazards of each. Reagent Function Health hazards 1. FAA 2. Ethyl alcohol 3. Xylene 4. Acetone 5. 10% formalin 6. paraffin 7. Harris Hematoxylin 8. Mayer's albumin 9. Canada balsam 10. Distilled waterWrite the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.
- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Name all the reagents used in Gram-staining and their roles in the staining procedure.
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayAnswer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?