What is the purpose of flame sterilizing the inoculating loop or needle before and after using it? What is meant by ‘streak dilution’ technique? What is the purpose of placing the inoculated agar plate in an inverted position in the incubator?
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- What is the purpose of flame sterilizing the inoculating loop or needle before and after using
it? - What is meant by ‘streak dilution’ technique?
- What is the purpose of placing the inoculated agar plate in an inverted position in the
incubator?
Step by step
Solved in 2 steps
- Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate. Effect: Rationale:What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?
- How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample to/from an agar plate?What physical appearance (Solid, liquid, semi-solid) of following Media: Broth tube, agar slant tube, agar deep tube, and agar plate. What exactly am I testing when I Introduce an inoculum with a needle on an agar deep tube?Identify the method of sterilization/ chemical agent described This chemical agent is used as a standard for evaluating new chemical agents. This chemical agent is used as water disinfectant. A phenol-derivative that is less exotic and more potent than phenol. Considered the best antiseptic. Used as prophylaxis for ophthalmia neonatorum.
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeList 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?
- Topic: Spread-Plate Method Agar was not completely set prior to inoculating with the organism. Effect:Rationale:Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in an easiest manner. ThanklsIn paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using x-ray crystallography and infrared spectroscopy? Please include the principles and the details and type specifically for the antimicrobial agents of the methodology.