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What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how does it work?
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- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions: D2 Lamp (B) Detector Light Source (A) Sample What are the components labelled as (A) and (B)? Briefly explain the functions of them.What are the light sources used in UV-vis spectrophotometry.
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawWhat is Ultraviolet (UV) light ?What are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?
- How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?What is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).