What is the color result of tryptophan,tyrosine and phenylalanine in xanthoproteic test? Results and interpretation with reference
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- it's so confusing, can you specify what are they positive for? like Positive for Xanthoproteic test (letters)** Negative for Xanthoproteic test. (letters)** Negative for phenol group (letters)** Has the presence of phenol group. (letters)**What are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.Is phenol red test a efficient test for unknown intestinal bacteria?
- Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, lg/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure 1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, 1g/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…Of , Shigella flexneri and Shigella sonnei, which are positive and which is negative for phenylalanine deaminase test results?
- What is the purpose of Xanthoproteic test and explain the observation or rection for albumin and gelatin in terms of principle of xanthoproteic test.I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I BELIEVED THAT THIS IS " S. PYOGENES. SO I DID A BACITRACIN TEST ON IT. IMAGE 2 SHOWS BACITRACIN TEST IMAGE. ** PLEASE HELP ME IDENTIFY IF THIS IS S. PROGENES AFTER LOOKING AT ALL THE THREE. BACITRACIN SHOULD BE THE CONFIRMATION TEST. IF YOU THINK THAT THIS IS S. PYOGENES AFTER LOOKING AT THE BACITRACIN TEST, THEN PLEAE EXPLAIN HOW DID YOU FIGURE IT OUT FROM THE CONFIRMATION THAT THIS IS S. PYOGENESE FROM BACITRACIN TEST. NEED VALID REASON WHY YOU BELIEVE IT IS S. PYOGENESWhat is the clinical significance of ESR determination? Differentiate Wintrobe from Westergreen regarding accuracy of the test in a tabulated order. NOTE: Kindly asnwer all the questions. Thank you!