The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonicanhydrase.H2O + CO2--> HCO3 + H+Carbon Dioxide Concentration(mmol L-')1.252.55.020.01/Velocity(M'sec)36 x 10³20 x 10³12 x 10³6 x 10³[H. DeVoe and G. B. Kistiakowsky, J. Am. Chem. Soc. 1961 83 (2), 274-280.]Use Excel (or similar) to generate a Line Weaver-Burke plot, then use the equation for a linear-fitted trend line to calculate the substrate affinity of CO2 for carbonicanhydrase. Answer in units of mM.Enter numerical values only, no letters or symbols. Round answer to 2 significant figures.

The substrate affinity of CO2 for carbonic anhydrase or Km is found to be 10 mM.Explanation:Step 1:…

Answered: The kinetic data in the following table…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The energy of activation for the reaction 2 HI – H2 + I2 is 180. kJ•mol-1 at 544 K. Calculate the rate constant using the equation k = Ae-EalRT. The collision diameter for HI is 3.5×10-8 cm. Assume that the pressure is 1.00 atm. 4.0 4.510e-27 X M-1.s-1
Consider the following reaction at 25°C with the ΔG°’ = +1800 J/mol for the forward reaction.The molar concentrations at the beginning of the reaction were [A] = 19 mM and [B] = 10 mM.After 1 hour, the concentrations were [A] = 16 mM and [B] = 13 mM. Calculate the ΔG of the reaction at the 1 hour timepoint. Please round to 1 decimal point.Gas constant = 8.315 J/mol K
At 298 K the standard enthalpy of combustion of sucrose is −5797 kJ mol−1 and the standard Gibbs energy of the reaction is −6333 kJ mol−1. Estimate the additional non-expansion work that may be obtained by raising the temperature to blood temperature, 37 °C.
In 2011, researchers showed that hypochlorous acid (HClO) generated by white blood cells kills bacteria. Calculate the percent dissociation of (a) 0.40 M HClO; (b) 0.035 M HClO (Ka = 2.9x10-8).
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Calculate ΔG° (answer in kJ/mol) for each of the following reactions from the equilibrium constant at the temperature given. (d)CoO(s)+CO(g)⇌Co(s)+CO2(g) T=550°C Kp=4.90×102 (e)CH3NH2(aq)+H2O(l)⟶CH3NH3+(aq)+OH−(aq) T=25°C Kp=4.4×10−4 (f)PbI2(s)⟶Pb2+(aq)+2I−(aq) T=25°C Kp=8.7×10
Direct methanol fuel cells (DMFCS) have shown some promise as a viable option for providing "green" energy to small electrical devices. Calculate E° for the reaction that takes place in DMFCS: CH3OH(I) + 3/2 02(g) → CO2(g) + 2 H20(1) Use the following values. AG°H,0(1) = -237 kJ/mol AG°O2(g) = 0 kJ/mol AG°CO2(9) = -394 kJ/mol AG°CH3OH(I) = -166 kJ/mol. E° = V
Calculate AG for the below balanced redox reaction as written. You may find a list of standard reduction potentials useful. 3 12 (s) + 2 Al (s) → 61 (aq) + 2 Al3+ (aq) O-4.24 x 102 kJ/mol O-6.48 x 102 kJ/mol O -2.12 x 102 kJ/mol O-1.27 x 103 kJ/mol O-6.37 x 102 kJ/mol
Use the Michaelis-Menten equation to determine the velocity of reaction when: • [S] = 15.0 mM Vmax = 94.0 umol/mL sec • Km = 4.00 mM Velocity of reaction = umol/mL sec
Find the initial velocity for an enzymatic reaction when Vmax = 6.5 x 10–5 mol.sec–1, [S] = 3.0 x 10–3 M, and KM = 4.5 x 10–3 M.
Given the oxygen dissociation curves, which of the following statements are correct? Which residue in HbA β do you think contributes the most to the increased interaction between HbA aand 2,3‑BPG?
Calculate the equilibrium constant for the reaction glucose-1-phosphate + H2O → glucose + H2PO4− at pH 7.0 and 25°C (ΔG°′ = −20.9 kJ · mol−1).

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