The following 2 compounds are found to be white solids. Which of the following techniques can be used to separate them OH + I. Liquid-Liquid extraction with an aqueous NaOH solution Liquid-Liquid extraction with an aqueous HCl solution Silica Gel Chromatography Distillation II. III. IV. I and II I and III I and IV II and III II and IV III and IV
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- 1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than component B. The following result is obtained. Calculate the Rifor each component and label the identity of each Frond (3-0,4)CM3D2.6cmr A (2-6-04 m = 2.2cm B C67-0.ycm=0.3cm RF (A= 2.2cm = 0.85 2. Lem spot. 2 39で Solvent Front A0-2.6m %3D 3cm Spoting Line Rf (8- 0.3cm 2. Scm = 0.12 %3D 2. Which one is the more polar component? Explain IS morC polo Because its Rf Is low 3. If a more polar solvent (mgbil phase) is used for the above experiment, the result will be: O Both component A and Component B will travel further up the plate-Distance may verur b) Only Component A will travel further up the plat c) Only Component B will travel further up the plat d) They will travel on the plate the same distance as when a less polar Choose one of the answers and explain 4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) is used for…Chemistry In a reversed phase liquid chromatography separation, what trend in k’ (capacity factor), α (selectivity or separation factor), and R (resolution) is expected as the ratio of methanol to water decreases? Explain the reason for this trend.1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than component B. The following result is obtained. Calculate the R,for each component and label the identity of each spot. 2 Solvent Front- A0-2.6km -3CM B00.7km Spotting Line 8.49 2. Which one is the more polar component? Explain 3. If a more polar solvent (mobil phase) is used for the above experiment, the result will be: a) Both component A and Component B will travel further up the plate b) Only Component A will travel further up the plat c) Only Component B will travel further up the plat d) They will travel on the plate the same distance as when a less polar Choose one of the answers and explain 4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) is used for stationary phase. An unknown liquid sample is analyzed using paper chromatography using solvent X as the mobile phase. One spot is observed after the plate is…
- 12.You want to separate compound A from compound B by flash chromatography. The following are the results of running these two compounds in different hexanes/ethyl acetate systems. Choose the best solvent system for the separation A from B and which one elutes column firstly? 3:1 121 bexancsethyt te a.Solvent system (3:1) – First eluted compound (B) b.Solvent system (6:1) – First eluted compound (A) c.Solvent system (12:1) – First eluted compound (A) d.Solvent system (6:1) – First eluted compound (B) e.Solvent system (3:1) – First eluted compound (A)[7] Which of the following are necessary steps in performing paper chromatography? I. Saturate the chamber with the vapor of the mobile phase II. Allow the chromatogram to touch the sides of the beaker III. Place the spots at the same height as the solvents IV. Spot the samples 2 cm apart from each othertrue or false: You are using an HPLC method based on partition chromatography to separate four analytes. Each analyte has the same equilibrium constant value that describes its interaction and concentration in the stationary and mobile phases.
- a. Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used? HPLC: 15% H₂O, 85% CHCN, CIS Revane Phase, 100 mm long, 5pm particles (1) Area (1)-14750 Arta (2)-53538 MIN HPLC: 59% 10,49% CHCN, CIS Reverse Phase, 100 m long, 5 pm particles (2) Mes (1)-2256 A(2)-9536 A(3)-25638 13HArrange in INCREASING Rf if the mixture underwent paper chromatography with the same solvent system. Explain how you get the answer I. 2-chloropentaneII. pentanoic acidIII. cyclopentaneIV. pentanal a. II = IV < III < I b.II < IV < I < III c.I < II < III < IV d.III < I < IV < IIWhich of the following is best to use to separate butanol from the volatile mixture? a. Affinity chromatography Ob. Liquid chromatography Oc. Size exclusion chromatography O d. Gas chromatography
- (7) Answer the following questions with the choices (a-d): (a) "Affinity" (b)"Adsorption" (c)"Size-exclusion" (d) "Partition"Rank the following compounds in terms of decreasing theoretical retention factors if a reverse-phase chromatography set-up was used. H H H H H H TTT 1.Н-С-С-С-О-н ii. їїн-с-с-с-" I ТТ ННН H OH OH 0 а. jijii 0 b. >> О с. iiiiii 0 d. >>Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (i) (ii) (iii) (iv) 0.01 TO 0.8 1.2 0". BEO - 2.0 1757 $122. 2.8 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?