The diagram below shows an outline of the aminotransferase mechanism that skips the specific steps that show how electrons flow when a Schiff base is formed or is hydrolyzed. Using the mechanistic details given below A. Draw the mechanism for Enzyme- PLP Schiff base formation using pyridoxal and the lysine amine group using arrow to indicate electron flow.B.Draw the mechanism for hydrolysis of the Schiff base to form the a-keto acid, which is the reverse of the first reaction. Stage 1Lys residue0,PO-HO+0,PO-step /Enz#tep 2step 3NEnzyme-PLPSchiff baseRPyridoxine(vitamin B.)Enzyme-PLPSchiff baseOHCHIE-mAminoacidH(rate determining)=0 + E¬NH₂-E"NH₂OH 0,90AminoacidH-CooR-C-COO"NH₂[H*0,90-HосногоNH₂Lys residue*0,PO-Pyridoxalphosphate (PLP)E-N-EHR> R-CO-HEAmino acid-PLPSchiff base(aldimine)(CH₂NH₂OHAmino acid-PLPSchiff base(aldimine)Stage 2=Z-m0,90-N%₂*0,90-+ H₂ONH₂0,PO-ExpedPyridoxamine phosphateKetimineSchiff basea-Keto acidKetimineFigure 17.16Biochemistry, First Edition, Copyright © 2017 W. W. Norton & Co., Inc.Other reagents can add as well. One group of compounds that adds is amines, substituted ammonia molecules with the general formula RNH₂. The reaction mechanism is very similar to those shownfor the addition of H₂O or alcohols. It is acid catalyzed. The first step of the mechanism generates a carbocation. This reacts with the lone pair of electrons on the nitrogen in the amine. The unstableproduct of this step is stabilized by the loss of an equivalent of water from the molecule, to form a Schiff base as shown below.a-Keto acidC-cooA. show how this formsStage 1Lys residueEnzEnzyme-PLPSchiff baseB. show how the a-keto acidformsNH₂0,PO-ChalaNH₂O,PO-Ketiminea-keto acid-cooPyridoxamine phosphate

The condensation reaction between an amine group (nitrogen bonded to two or more alkyl/aryl groups)…

The diagram below shows an outline of the aminotransferase mechanism that skips the specific steps that show how electrons flow when a Schiff base is formed or is hydrolyzed. Using the mechanistic details given below A. Draw the mechanism for Enzyme- PLP Schiff base formation using pyridoxal and the lysine amine group using arrow to indicate electron flow. B.Draw the mechanism for hydrolysis of the Schiff base to form the a-keto acid, which is the reverse of the first reaction.

The diagram below shows an outline of the aminotransferase mechanism that skips the specific steps that show how electrons flow when a Schiff base is formed or is hydrolyzed. Using the mechanistic details given below A. Draw the mechanism for Enzyme- PLP Schiff base formation using pyridoxal and the lysine amine group using arrow to indicate electron flow. B.Draw the mechanism for hydrolysis of the Schiff base to form the a-keto acid, which is the reverse of the first reaction.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter21: Heparin Infusion Calculations

Section: Chapter Questions

Problem 13SST

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