Solute Blue dextran Molecular Weight of solute Log' of the # Na2CrO4 2,000,000 Alkaline phosphatase Cytochrome C| 12,400 86,000 162 Log (Molecular wt.) 6.3 4.93 4.09 2.20 Elution Volume Ve (ml) 0.5M) AI 65 -6 2:3ML 3.5Ml 1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue dextran. Why? V- 3.14 r²h Vt = Measure inner radius of the column, r = Measure the Height of the column bed, h= 2. Calculate the total volume occupied by the column Vt using following equation and data provided,
Solute Blue dextran Molecular Weight of solute Log' of the # Na2CrO4 2,000,000 Alkaline phosphatase Cytochrome C| 12,400 86,000 162 Log (Molecular wt.) 6.3 4.93 4.09 2.20 Elution Volume Ve (ml) 0.5M) AI 65 -6 2:3ML 3.5Ml 1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue dextran. Why? V- 3.14 r²h Vt = Measure inner radius of the column, r = Measure the Height of the column bed, h= 2. Calculate the total volume occupied by the column Vt using following equation and data provided,
Chapter7: Statistical Data Treatment And Evaluation
Section: Chapter Questions
Problem 7.26QAP
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Transcribed Image Text:Solute
Blue dextran
Molecular Weight
of solute Log' of
the #
Na2CrO4
2,000,000
Alkaline
phosphatase
Cytochrome C| 12,400
86,000
162
Log (Molecular wt.)
6.3
4.93
4.09
2.20
Elution Volume Ve (ml)
0.5M) AI
65 -6
2:3ML
3.5Ml
1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue
dextran. Why?
V- 3.14 r²h
Vt =
Measure inner radius of the column, r =
Measure the Height of the column bed, h=
2. Calculate the total volume occupied by the column Vt using following equation and data
provided,
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