Experiment:Page 6 de can exist stably in either a haploid or a diploid state. A haploid S. cerevisiaemes. When certain haploids come into contact, they fuse their cell walls andmembranes, followed by the fusion of their nuclear membranes. The single nucleus now has 32chromosomes, 16 from each parent strain, and is thus a diploid.Haploid yeast strains divide mitotically to give rise to haploid progeny, and diploid strains dividemitotically to give rise to diploid progeny. Certain haploids can fuse to form diploids. Haploid S.cerevisiae exists in two "mating types," called a and a Mating occurs only between a and a cells; nomating occurs between cells of identical mating type.We have a collection of eight a haploid mutant strains and eight & haploid mutant strains of yeastunable to synthesize tryptophan (trp). These will be combined (mated) in all possible combinationsto yield diploid strains. If the diploids can grow on minimal medium, complementation occurredbetween the genomes of the two haploids, and the mutations are thus in different complementationgroups. If the diploids fail to grow on minimal medium, then no complementation occurred and themutations lie within the same gene, or same complementation group.Mutant haploid strain aMutant haploid strain aMatea/a DiploidGrowth on minimal medium?NoYesSame complementation group Different complementation groupsExperimental outlineThe eight a and eight & trp mutant strains are combined in all possible a/a pairs on a plate of richmedium according to the following grid.a (columns)1/1 1/2 1/3 1/4 1/5 1/6 1/7 1/82/2 2/3 214 215 216 217 218313 314 315 316 317 318414 415 416 417 418a (rows)5/5 5/6 5/7 5/86/66/74/87/7 7/68/8 The patches are allowed to grow overnight during which the haploids fuse into diploids. A very lightinoculum of each patch is then transferred to an unsupplemented minimal plate in the same patternas above, and the plate is incubated for two days at 30°C. Growth or absence of growth is thendetermined for each patch.678a MasterMating plateRich medium123456 7 8a MasterTransfer24 hr laterOHComplementation plateMinimal mediumScore results 48 hr laterQuestion 11: If mutant haploid strain is mixed with a wild-type haploid strain, how manychromosomes will their progeny have?

Name: Experiment:Page 6 de can exist stably in either a haploid or a diploi
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Description: Experiment:Page 6 de can exist stably in either a haploid or a diploid state. A haploid S. cerevisiaemes. When certain haploids come into contact, they fuse their cell walls andmembranes, followed by the fusion of their nuclear membranes. The single

Haploid and diploids An organism is said to be haploid when it consists of a single copy of a…

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Question 11: If mutant haploid strain is mixed with a wild-type haploid strain, how many chromosomes will their progeny have?

Biology: The Dynamic Science (MindTap Course List)

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ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter30: Fungi

Section: Chapter Questions

Problem 14TYK: Design an Experiment Experiments on the orange bread mold Neurosporacrassa, an ascomycete, were...

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