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- 1. What is Du? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.7 2. Why do we need test for Du when weak or no reaction is obtained in Rh typing? 3. What is required to demonstrate the presence of cells carrying a weak D antigen? 4. Explain the mechanism behind the existence of Du. 5. Cite possible situations in which an attending physician would request for an emergency screening for the presence of "D" antigen.1. What is Du? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.7 2. Why do we need test for Du when weak or no reaction is obtained in Rh typing? 3. What is required to demonstrate the presence of cells carrying a weak D antigen? 4. Explain the mechanism behind the existence of Dº. 5. Cite possible situations in which an attending physician would request for an emergency screening for the presence of "D" antigen.5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined by MTT assay. a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square Dilution factor: 5 49
- 1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B1. State the significance of compatibility test in transfusion medicine? 2. What is a major crossmatch? A minor crossmatch?
- Give typing answer with explanation and conclusion 8. Considering the principle of the Neutral Red bioassay, describe, in details, the expected appearance of the cheek/buccal cells if exposed to a toxic substance such as cigarette ash or possibly any other type of toxic substance.2.4. Preparation of a Calibration CurveStandard Bovine Serum Albumin (BSA) solutionStock solution: 2.00 mg/mLStandard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSADiluent: distilled water a. on the picture b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL ofdistilled water. Mix using a vortex mixer.c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of theremaining concentrations following the sample calculation. Table 2.4. Volumes needed for the preparation of the BSA standard solutions. Concentration to beprepared, mg/mL Volume of 2.00 mg/mLstock solution needed, μL Volume of distilledwater needed, μL 0.00 0 1000.00 0.125 62.5 937.5 0.25 0.50 1.00 1.50 2.00 please complete the given table with the correct computation like in the pictureExplain the advantages of using cell based assays for drug screening? Pls no chat gpt
- 22:23 1O 000 · 11:24 A9 OB1 r ll l 52% . +964 782 734 3923 2m541139927815107... Patient Encounter Part 3 The pretreatment workup is summarized below. Pathology: 47-year-old female with new diagnosis of infiltrating intraductal adenocarcinoma involving the left breast and regional node. Further tests on tumor samples indicated ER (8%), PR (negative), HER2 (negative), Ki-67 (72%), and grade (poorly differentiated). Intrinsic subtype (luminal B, HER2-negative). Radiology: FDG-PET/CT indicated a 5.3 x 2.5 cm mass in the left breast which appeared to extend to the epidermis of the skin; one node in the left axilla was also involved with tumor. No other evidence of distant disease was visualized. Laboratory: CBC, liver, and kidney function tests WNL, alkaline phosphatase and calcium are normal also. Stage: IB (T, N, M,) List the most important prognostic factors in this patient with newly diagnosed breast cancer. Assess the patient's level of risk for relapse. 50 SECTION 16 | ONCOLOGIC…BiDil® is indicated for the treatment of heart failure as an adjunct to standard therapy in self-identified black patients to improve survival, to prolong time to hospitalization for heart failure, and to improve patient-reported functional status Question: The results of BiDil® clinical trials were compelling…Expound on the results. Question : What role did FDA’s approval resulted in BiDil® being a great example of pharmacogenomics/pharmacogenetics in the U.S.?1. Construct one plot including the data for the WT and the MC4R mutants presenting the differences in membrane localisation. 2.Obtain approximate Emax and EC50 values from the graphs. 3.Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring only total PE fluorescence? 4.Explain the effect that each mutation causes in the function of MC4R, and how they can be linked to disease.