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- You want to prepare a 15 mL gel containing 0.8% (weight /volume) agarose. How much agarosedo you need to weigh to prepare the solution? Show all your calculations.Hint: Write down the units for 0.8% knowing that agarose is a powder that is mixed with aliquidYou have just used up the last of a 1.2 % m/m solution of agarose in water. You require another 100mls. How many grams of Agarose do you require? Note 1ml of water=1g What is the % w/v of a 100ml solution containing 5g of NaCl?You have 10 mg/ml ethidium bromide solution. If you add 5 µl of this to 50 ml of agarose gel solution, what will be the final concentration of ethidium bromide in the gel solution in mg/ml?
- As a part of a clinical study, a pharmacist is asked to prepare a modification of a standard 22 g package of a 2% mupirocin ointment by adding the needed quantity of mupirocin powder to prepare a 3% w/w mupirocin ointment. How many mil- ligrams of mupirocin powder are required?A pharmacist receives a prescription for 60 g of a 0.75% w/w bexarotene gel. How many grams each of a 1% w/w bexarotene gel and gel base must be used?The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- One tenth mL of a protein solution was diluted with 2.5 mL of water. The A280 of the diluted solution was 0.25. How many mL of the original protein solution and water should be mixed to make 1.0 mL of solution of A280 = 0.80?How many moles of EDTA (C10H16N2O3) do you have in 785µl of a 74MM EDTA solution (Mr(C10H16N203) = 292.24)? Consider all digits of your Givens significant. Round results to the appropriate number of Significant Digits. The number of moles is mmol.If a pharmacist added each of the following to 22-g packages of 2% mupirocin ointment, what would be the percentage strengths of the resulting ointments: (a) 0.25 g mupirocin powder and (b) 0.25 g of nonmedicated ointment base? (answer to two decimal places). answers: (a) 3.10% w/w (b) 1.98% w/w
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