how to find out the test is differential or selective in the microbiology lab test?

Q: What is an example of an experimental approach for determining whether a beef burger contains a food…

A: Beef burgers served rare or undercooked may contain harmful bacteria that could cause food…

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…

A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…

Q: How would you draw out a step in an experiment that included the following? Please help, I am so…

A: Horizontal gene transfer (HGT) is largely responsible worldwide for increasing the incidence of…

Q: You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria…

A: The bacteria can grow only in the favourable conditions that is when there is optimum amount of pH,…

Q: What is the difference between culture-based technique and culture-independent techniques based on…

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.

A: Protein is a nitrogenous organic macromolecule that is essential to human health. It is responsible…

Q: Do we have to do an antibiotic sensitivity test to ensure the result? and what is the type and name…

A: Antibiotic Sensitivity Test:  Antibiotic sensitivity testing, often known as antibiotic…

Q: Why each bacterium must be tested separately?

A: Antibiotics are chemical substances that kill or inhibit or prevent the growth of microbes. They are…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: hy do we use broth for enrichment, but plates for isolation and identification?

A: What do you think of when you hear the word broth? Maybe it is a delicious liquid made from chicken,…

Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?

A: Answer:  Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…

Q: Give the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions…

A: Antibiotic discs are used in microbiological studies to observe the effects of various antibiotics…

Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…

A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…

Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…

A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…

Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…

A: Microbiology is the branch of science which deals with the study of different types microorganisms…

Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…

A: Dilution is the process of making a solution weaker or less concentrated.

Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…

A: Note: I can't provide you with pictures. But i will explain you functions of these apparatus.…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…

A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…

Q: What is the principle involved in the Acrolein test? What is its purpose?

A: Qualitative tests are the chemical or physical tests involved in identification of certain…

Q: what are the important principle in invertase test?

A: To determine enzymatic activity, the amount of substrate remaining or the amount of product created…

Q: what is the culture media for gram-positive bacteria?

A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…

Q: Why are traditional tests such as metabolic test not always used to ID clinically important…

A: Traditional methods for identification of microbes include phenotypic tests or biochemical tests…

Q: What is the importance of using a test/control organism in describing the cultural characteristics…

A: The food materials on which the organism is developed is called culture medium and the development…

Q: What are the examples of organisms that belong to the positive test in in-dose test?

A: Tryptophan is an amino acid that can undergo deamination. Indole is generated by reductive…

Q: How can a suspect bacterium be fingerprinted?

A: DNA fingerprinting is a technique for detecting a high number of minisatellites in the genome at the…

Q: What is the principle behind invertase test

A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…

Q: Why does fixation of the positive dye help bacteria stick to the slides?

A: Dyes in microbiology are for staining cells; they are used based on the chemical properties and the…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: What is the proper way of Modified Allen test?

A: Allen test are the tests used to assess the arterial blood supply of the hands. This test is used…

Q: What is the main advantage of using the slide culture technique for identifying molds?

A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…

Q: Agar was not completely set prior to inoculating with the organism. Effect: Rationale:

A: Bacterial cultivation requires specific types of medium that can provide some of the basic…

Q: In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For…

A:

Q: How can moist heat sterilization can sterilize antibiotic solution?

A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…

Q: What is the coliform test when is it used? What is its purpose? How does it function? What are the…

A: All water that is naturally exposed to the atmosphere, such as rivers, lakes, and reservoirs. It is…

Q: How does the agar dilution method differ from streaking toobtain isolated colonies?

A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…

Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…

A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…

Q: What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and…

A: The ideal colony size is found between 30 and 300. The CFU/ml is calculated by: (Colony × Dilution…

Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…

A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…

Q: You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.…

A: In serial dilutions, dilutions are multiplied at each step. We can calculate dilution by dividing…

Q: Which test tube is the control group?   Test tube 1: 5 Hydrogen peroxide only Test tube 2: 5…

A: The scientific method involves understanding and solving the phenomena in the universe. The…

Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…

A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…

Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…

A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…

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