) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE
Q: For each V (mmol/min), determine the value of [S] (mM) if Vmax = 5 mmol/min. Show your calculations....
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question s...
Q: According to the configuration of the isostatic, syndiotactic and atactic polymers. Define each of ...
A: TACTICITY :- The steric arrangement in polymers is called tacticity.
Q: Define each of the following metabolic processes and write notes on importance and regulation of ea...
A: Urea cycle is known as Krebs-Henseleit urea cycle. As ornithine is the first member of the reaction...
Q: Answer the following: This type of lipid is a complex mixture of esters of long-chain carboxylic ac...
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
Q: At a replication fork, which strand is synthesized discontinuously? O lagging O leading
A: Replication is an essential process taking place in all living organisms that ensure the maintenance...
Q: a. Identify the regulatory process an%3; b. Explain how this process works
A: Enzymes are biocatalyst. They act on a substrate and product is formed. Enzymes increases the speed...
Q: Purification of a protein of unknown structure has been achieved. The natural protein has a molecula...
A: Proteins are polymers of amino acids and form primary structure. It can form secondary, tertiary and...
Q: How to write a pre-lab preparation: lab notebook procedure(Density). And post-lab notebook (Density)
A: Before performing any chemical experiment in a lab, it is important to know how to write the Lab not...
Q: Indicate the contrast statements of the following chromatographic techniques of separation. A. A sep...
A:
Q: Suppose a mutant Fatty Acid synthase only synthesizes a FA 14 Carbons long. How many ATP will the sy...
A: Acetyl CoA is a hybrid molecule that is involved in a variety of metabolic processes in the body. As...
Q: An antibody (Ab) can bìnd to its antigen binding site (B) with a high affinity (large negative AG°)....
A: The antibodies bind to its antigen via non n covalent interactions like hydrogen bonding, hydrophobi...
Q: Carbon is particularly well suited to be the backbone of organic molecules because (a) it can form b...
A: Carbon is a chemical element with an atomic number of 6. Carbon is non-metallic and has four electro...
Q: Describe the growth cycle of a bacterial population from the moment when it is inoculated into a cul...
A: When singe cell colony of bacteria is inoculated into a growth media full of nutrient, they exhibit ...
Q: For each of the following genotypes, explain how mutation (identified by a (-) will affect the organ...
A:
Q: how/why you will characterize the proteins (whether impurities or dsRed.m1) as it comes off the DEAE...
A: Diethylaminoethyl cellulose (DEAE-C) is a positively charged, hydrophilic, weak anion exchange type ...
Q: Phospholipids spontaneously form a bilayer in an aqueous solution. Why do the heads of the phospholi...
A: The basic material for studying biological membranes has been red blood cells. Lipid and protein are...
Q: I. Name the following carbohydrates using the proper way of naming/IUPAC system. a. Erythrose b. Lyx...
A: Carbohydrates are one among the 4 biomacromolecules. They are called hydrates of carbon as their gen...
Q: Consider the following metabolic pathway and answer the questions. CH,OPO, CHOPO, H--OH HO-C-H H-C-O...
A: Hi, thank you for posting the question on Bartleby. As per the guidelines, we can answer up to three...
Q: Which functional site is found in the small subunit of the ribosome? peptidyl transferase centre O d...
A: Ribosomes, huge ribonucleoprotein particles with three RNA molecules and more than 50 proteins, are ...
Q: In full details Define each of the following and explain the role of each in named cells: i. Thermo...
A: I) Thermogenin: Thermogenin or uncoupling protein which is now known as uncoupling protein 1, or UCP...
Q: Why can't humans digest cellulose while ruminants can? What is the difference between the two organi...
A: Cattle, sheep, and goats are examples of ruminant livestock. Ruminants are hoofed mammals with a dig...
Q: Defintion of Vision's Chemistry?
A: -Light is absorbed by a pigment in a photoreceptor cell (by a dye in the eye), and the resulting pho...
Q: What is the one ingredient which turns up in all of the formulas and where does it come from? How an...
A: Tetrodotoxin: Tetrodotoxin is a low-molecular-weight neurotoxin which is a wide variety of taxa. It...
Q: 1. List as many as possible blood bank serologic tests that are affected by hemolyzed samples (list ...
A: Hemolysis: It is the breakdown of red blood cells and there are a few reasons why blood can be hemo...
Q: Propose one amino acid substitution that would disrupt the leucine zipper structure and explain why
A: Leucine zipper structure is formed by leucine residues and these leucine residues are held together ...
Q: sugar
A: polysaccharides : it is a polymer made up of many sugar subunits, called monosaccharides . Polysacch...
Q: How to use the sterile filter? Show the different sizes and specifications of sterile filters.
A: Sterilization is the method that is used to eliminate all forms of life and biological agents presen...
Q: What name is given to the assembly of proteins that carry out DNA copying at a replication fork? O c...
A: Every time cells divide, eukaryotic genomes are duplicated with near-perfect fidelity. The circumsta...
Q: The following RNA polymer is added to an E. coli extract, where it can be translated in all three po...
A: A reading frame is the sequence of nucleotides in a nucleic acid (DNA or RNA) molecule read into a s...
Q: proteins are deamidated in an aqueous solution to produce ammonium and aspartate, respectively, from...
A: Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different m...
Q: Summary Chart Macromolecule Monomer Polymers Bonds Functional Groups Examples CH2OH CH2OH он - CH,-C...
A: Macromolecules are the molecules of high molecular masses. These are composed numerous small molecul...
Q: discuss the implications of having a good culture medium for the diagnosis of plant diseases?
A: Plant diseases are caused by different reasons ranging from pest infestations till waterlogged roots...
Q: What are the advantages and disadvantages of viable cell count and the turbidimetric methods?
A: In turbidimetric determination of cell growth ,after inoculation of the bacterial culture the optica...
Q: an emergency service. He was very slim and smelled acctone. Blood test results showed the following:...
A: Ketone bodies include 3-hydroxybutyrate (3-OHB), acetoacetate, and acetone. They are the fuel substr...
Q: say you have been asked to determine the molecular structure of a soluble protein with roughly 500 a...
A: Determining the structural basis of protein in a molecular or even atomic level would enable us to u...
Q: Which of the following cofactors will eventually dissociate from their enzyme polypeptide given enou...
A: Enzymes are usually composed of proteins that contain active sites for the substrate binding. It wor...
Q: You are working in a research lab that is surveying the CFTR gene sequence in humans to identify new...
A: A synonymous mutation is mutation in the DNA sequence that leads to change in the codon for amino ac...
Q: What name is given to the assembly of proteins that carry out DNA copying at a replication fork? tra...
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...
Q: Define the term isomer and distinguish among the three principal isomer types.
A: Molecules are composed of atoms. Atoms are the smallest particles of an element that can exist. Atom...
Q: Name some techniques/approaches (name, describe the major steps, how to analyze/interpret results) ...
A: Proteins: Proteins are polymers that contain hundreds or even thousands of amino acids that are lin...
Q: Ketosis in Dairy Cattle: 1. Explain why propionate can contribute to the next synthesis of glucose b...
A: Ketosis in dairy cattle is a metabolic disorder appears with increased energy demand due to starvati...
Q: What are the different types of solutions according to their concentration? What is its effect of ea...
A: Solutions: A solution is a homogenous mixture that contains a similar kind of substance (solute) di...
Q: When you are served with the dessert, you had an emergency call from home and you need to return ho...
A: From the production of food to consumption of food it goes through several steps which include m...
Q: A peptide has the following amino acid composition: 2 Met, 2 Phe, 2 Glu, 1 Arg, 1 Lys, 1 Val, 1 Leu,...
A: Small molecules called amino acids to form the building blocks of proteins. In chemistry, an amino a...
Q: Read through each scenario. Under the scenario, write which lab safety rule is being broken and indi...
A: Hi! Thank you for the question, as per the honor code, we are allowed to answer the first 3...
Q: You have 10 mg/ml ethidium bromide solution. If you add 5 µl of this to 50 ml of agarose gel solutio...
A: Gel electrophoresis is a laboratory method for separating DNA molecules depending on their size and ...
Q: In Eukaryotes, some hydrogen bonds are formed in between amino acids of the histones and phosphodies...
A: DNA and RNA are made up of long chains of nucleotides and ribonucleotides bases. Sugar molecule...
Q: Both triglycerides and prostaglandins can contain unsaturated fat. O True O False
A: Triglycerides (triacylglycerols) are stored form of fat. Triglycerides are composed of three fatty a...
Q: importance in familiarizing and knowing the uses of common laboratory
A: The question is all about the knowledge and familiarity of common laboratory equipments that is used...
Q: 5' AU Py U A AGGCC G. UGGC G C G GGG What modified nucleoside base is indicated by the arrow? dihydr...
A: The answer of the above question is : Pseudouracil (Ψ). Pseudouridine (psi- Ψ)is an isomer of the nu...
With the aid of diagrams, explain the processes and principles of each of the following
methods:
(a) Gel filtration chromatography on Sephadex G50
b.SDS-PAGE
Step by step
Solved in 3 steps with 2 images
- With the aid of a diagram, explain the principles of gel filtration chromatography.Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.
- Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a hospitalized patient with a urinary tract infection. The organism produced colorless colonies on MacConkey agar and gives the following biochemical reactions: PAD – positive H2S – positive positive Citrate – positive Ornithine positive Lysine – negative Indole - negative Urease –A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
- based on the chart answer the following: 1. which proteins will be most excluding use gel filtration chromotagrpahy 2. which proteins will migrate closer to the anode using SDS- PAGE 3. which protein will have the most net positive charge at a ph of 7.4What is the basic principle of GPC (gel permeation chromatography)?As a public health engieer you have been tasked wiyh designing a plug flow reactor for the disinfection of Giardia lamblia using either free chlorine and monochloramine. The objective is a three log reduction in the concentration of Giardia lamblia. The kinetics of chlorine decay can be described by a first order coefficient of 0.1 d-1. It is also given that the appropriate contact time, t10, equals the hydrualic residence time (HRT or td) of the plug flow reactor. Question: At a temperature of 5oC and a pH of 7.0, what is the volume of the PFR if the flowrate of the water to be disinfected is 1000 L/d for chlorine and monochloramine Disinfection data in the table below can be used.
- briefly explain the principle behind this technique; gel-filtration chromatographyConstruct a table showing the composition, advantage and disadvantage of these Platelet Diluting Fluids (1) Tripotassium EDTA and 1% Ammonium Oxalate and (2) Rees and Ecker Diluting Fluid.Erysipelothrix rhusiopathiae is a gram positive bacilli that survives in water contaminated by a sick animal’s feces or urine, soil, or plant material. It is known for causing zoonoses, and is typically associated with occupational exposure to animals. There are a few tests commonly used that help to differentiate the organism. Indicate the results for the following reactions: Catalase: TSI: Growth in gelatin: